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Design of a CRISPR-Cas system to increase resistance of Bacillus subtilis to bacteriophage SPP1
It is found that the heterologous CRISPR-Cas system is functionally active in B. subtilis and provides resistance against bacteriophage SPP1 infection and the high efficiency of the acquired immunity against phage could be useful in generation of biotechnologically important B.subilis strains with engineered chromosomes.