Lin-chang Chiang

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NIH3T3 cells grow in a serum-free basal nutrient medium supplemented with fibronectin, transferrin, insulin, epidermal growth factor (EGF) and high density lipoprotein (HDL). The individual omission from the serum-free medium of insulin, EGF, or HDL results in greatly reduced cell growth. These growth-restrictive conditions can be used to select for cells(More)
The first example of an enzymatic synthesis of 2-carbamoyloxyethyl methacrylate by a lipase-catalyzed transesterification of 2-hydroxyethylcarbamate with vinyl methacrylate in organic solvent is reported. Using the lipase PS-30 from the Pseudomonas species under optimal conditions, a temperature of 50 °C and a solvent mixture (3:1) toluene/THF,(More)
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