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Morphine is used to treat pain in several medical conditions including cancer. Here we show that morphine, in a concentration typical of that observed in patients' blood, stimulates human microvascular endothelial cell proliferation and angiogenesis in vitro and in vivo. It does so by activating mitogen-activated protein kinase/extracellular(More)
The afferent and efferent connections of the dorsal tegmental nucleus (DTN) were studied in the rat using axoplasmic transport techniques. Horseradish peroxidase (HRP) and Fast Blue were injected stereotaxically into either pars centralis or pars ventromedialis of the DTN, two subdivisions of the nucleus with distinctive connections. The pars centralis is(More)
BACKGROUND MicroRNA-7 (miR-7) has been reported to be a tumour suppressor gene. However, whether it has a role in the growth of non-small-cell lung cancer (NSCLC) and what is its target involved in the tumour growth is still under investigation. METHODS NSCLC tissue sample, NSCLC cell lines and tissue microarray were investigated in this study. Total RNA,(More)
A culture of human blood outgrowth endothelial cells (BOECs) was established from a sample of peripheral blood and was transfected using a nonviral plasmid carrying complementary DNA for modified human coagulation factor VIII (B domain deleted and replaced with green fluorescence protein). BOECs were then chemically selected, expanded, cryopreserved, and(More)
A methodology for rapid isolation of neuroblastoma cells from marrow with metastatic neuroblastoma cells was developed using a cocktail of five antibodies and magnetic microspheres coated with secondary antibodies. Cells bound to microspheres were released by brief exposure to chymopapain, followed by repeated culture of released cells in serum-supplemented(More)
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