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We studied the intracellular total, oxidized and reduced glutathione levels in thymus and spleen rat lymphocytes cultured with or without Con A and 2-mercaptoethanol (2-ME). After 48 h culture, the total glutathione level decreased and the oxidized glutathione level increased in the two types of unstimulated and stimulated cells. In the presence of 2-ME,(More)
BACKGROUND Programmed death-1 (PD-1) costimulation acts as a negative regulator of T-cell responses to allografts. However, the role of the PD-1 pathway in xenotransplantation is not well defined yet. We have shown previously that human in vitro T-cell responses to porcine transfectants overexpressing PD-Ligand1 (L23-PD-L1 cells) are remarkably weak. In(More)
BACKGROUND T cells are known to participate in the response to tumor cells and react with cytotoxicity and cytokine release. At the same time tumors established versatile mechanisms for silencing the immune responses. The interplay is far from being completely understood. In this study we show contacts between tumor cells and lymphocytes revealing novel(More)
Glutathione is known to be an important parameter for ConA proliferative response of murine splenocytes. We studied the glutathione status of ConA-stimulated rat splenocytes during the early and late phase of the mitogenic response under low (FO2 7%) and standard (FO2 21%) oxygen concentrations. We determined the intracellular total, oxidized and reduced(More)
Time dependent response for hyperoxic exposure was determined in vitro on ConA proliferative response of rat splenocytes, peripheral blood mononuclear cells and thymocytes. The proliferative responses were evaluated after different lengths of hyperoxic exposure (12-72 h, FiO2 = 0.95). After 24 h oxygen exposure, the spleen cell viability assessed by dye(More)
Rat spleen cells were cultivated in 1 ATA pure oxygen. The mitogenic responses with Con A were evaluated at different time of exposure (12 to 72 hrs). There was a stimulation of the cells after 12 hrs of exposure, which diminished at 18 hrs, in spite of the fact that the viability of the cells remained unchanged till 24 hrs of exposure. The kinetics of the(More)
The in vivo mitogenic responses to lipopolysaccharide or concanavalin A by spleen cells of mice exposed to 20 ppm nitrogen dioxide (NO2) for 96 hr, were evaluated. [3H]Thymidine incorporation after addition of either mitogen, was significantly lower in spleen cells from acutely NO2-exposed mice (NO2 SC) than from control mice, although cell viability was(More)