Liljana Mitova

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The expression of GABA receptors (GABARs) was studied in frog and turtle retinae. Using immunocytochemical methods, GABA(A)Rs and GABA(C)Rs were preferentially localized to the inner plexiform layer (IPL). Label in the IPL was punctate indicating a synaptic clustering of GABARs. Distinct, but weaker label was also present in the outer plexiform layer.(More)
The effect of GABAergic blockade by picrotoxin on ganglion cells (GC) activity was investigated in perfused dark adapted eyecups of frog (Rana ridibunda). PT had diverse effects on the light responses of GC in contrast to its uniform potentiating effect on the amplitude of the ERG b- and d-wave. In some (n=32) of PT-sensitive ON-OFF GC the ON and OFF(More)
Perfusion with the ON channel blocker 2-amino-4-phosphonobutyrate (APB) of dark adapted frog eyecups not only abolished the ganglion cells' (GC) ON responses and the ERG b-wave, but markedly potentiated the OFF responses of ON-OFF and phasic OFF-GCs and the d-wave amplitude of simultaneously recorded local ERG. Glycinergic blockade by strychnine prevented(More)
The aim of the present work was to investigate the role of GABA and glycine, the two main inhibitory neurotransmitters in the retina, in the spectral sensitivity coding under conditions of light adaptation. To study this question, spectral sensitivity curves, based on the turtle ERG responses to stimuli of different wavelengths, were constructed. The(More)
A comparative study was made of the ERG b- and d-wave intensity-response functions before and after GABAergic blockade by means of 0.4 mmol l-1 picrotoxin. A wide range of background intensities, including part of the high photopic range, were used. The intensity-response functions of both the ERG waves fitted well to the Michaelis-Menten equation (V/Vmax =(More)
Superfusion with 200 microM 2-amino-4-phosphonobutyrate (APB) of dark and chromatically adapted frog eyecups caused marked potentiation of the ERG OFF-response (d-wave). Blockade of the glycinergic synapses by strychnine did not change this effect at all. Blockade of the GABAergic synapses by picrotoxin slightly diminished the effect of APB in(More)
1. Electroretinogram (ERG) and responses of single ganglion cells to 75 microseconds light flashes, presented on two different backgrounds, were studied. Additionally, stimulation with long lasting stimuli (ordinarily 5 sec ON-, 15 sec OFF-) was used. 2. 2-amino-4-phosphonobutyrate (APB) at a concentration of 450 microM on average was used to separate OFF-(More)
The ERG ON- (b-wave) and OFF-response (d-wave) to differently coloured stimuli was studied using a wide range of stimulus intensities in dark adapted turtle retina. The intensity-response curve of the b-wave showed saturation but that of the d-wave, decline in the high-intensity stimulus range. The curves of the relative spectral sensitivity of the ERG ON-(More)
The intensity-response (V/log I) function of ERG OFF response (d-wave) in dark and light adapted superfused frog eyecups was investigated before and after blockade of the retinal ON channel by 2-amino-4-phosphonobutyrate (APB). The V/log I function of the dark adapted d-wave had two distinct components, each of them consisting of an ascendent and descendent(More)