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Artemisinin is extracted from a traditional Chinese medicinal herb Artemisia annua L., which is regarded as the most efficient drug against malaria in the world. In recent years, attention has been paid to increase the artemisinin content through transgenic methods because of the low content of artemisinin in wild plants. In this article, three functional(More)
Artemisinic aldehyde Δ11 (13) reductase (DBR2) is the checkpoint enzyme catalyzing artemisinic aldehyde to form dihydroartemisinic aldehyde directly involved in artemisinin biosynthetic pathway. In the present study, DBR2 was employed to engineer the biosynthetic pathway of artemisinin in transgenic plants of Artemisia annua L. Seven independent transgenic(More)
Methyl jasmonate (MeJA) is one of the most potent elicitors that can induce over accumulation of many natural products including artemisinin in plants. The 12 known genes (HMGR, DXS, DXR, HDS, HDR, FPS, ADS, CYP71AV1, DBR2, ALDH1, ORA and ERF1) of terpene metabolism in Artemisia annua were dynamically analyzed at the transcriptional levels in the treatment(More)
The selection and validation of reference genes are essential for gene expression studies by real-time quantitative PCR. The genetic map of Artemisia annua L., a Chinese medicinal plant species producing anti-malarial artemisinin, has been reported. However, few reference genes of A. annua have been estimated for real-time quantitative PCR until now. In(More)
OBJECTIVE To study the relative expression of the genes involved in artemisinin biosynthesis in different tissues including roots, stems, leaves and flowers of Artemisia annua, and establish the relationship between gene expression and artemisinin accumulation, eventually leading to discover the mainly effective genes involved in artemisinin biosynthesis.(More)
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