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An innovative analytical approach based on the use of comprehensive two-dimensional liquid chromatography (LC×LC) is applied to obtain the profiling of phenolic compounds in different apple varieties. The method combines the use of hydrophilic interaction liquid chromatography in the first dimension and a reversed phase separation in the second dimension,(More)
In the present work, the phlorotannin composition of different Sargassum muticum samples collected at different locations along the North Atlantic coasts as well as the bioactivities related to these components were investigated. After pressurized liquid extraction, the samples collected at the extreme locations of a latitudinal gradient from Portugal and(More)
Phlorotannins are an important class of polyphenolic compounds only found in brown algae. The chemical analysis of these bioactive polyphenols is rather difficult due to the great chemical variability and complexity of the natural composition of these components in algae, forming large phloroglucinol polymers. In the present work, a new approach based on(More)
In this work, the development and optimization of a new methodology to analyze grape seed procyanidins based on the application of two-dimensional comprehensive LC is presented. This two-dimensional method involves the use of a microbore column containing a diol stationary phase in the first dimension coupled to either a C18 partially porous short column or(More)
Algae have been consumed for many years in Asian countries; at present, its consumption around the world either as food ingredient or directly as food commodity has increased considerably mainly due to the Asian influence and its cuisine, but also because the growing interest on the beneficial health effects of algae. Although several biological activities(More)
Profiling of the main metabolites from several licorice (Glycyrrhiza glabra) samples collected at different locations is carried out in this work by using comprehensive two-dimensional liquid chromatography (LC × LC) coupled to diode array (DAD) and mass spectrometry (MS) detectors. The optimized method was based on the application of a HILIC-based(More)
Grapevine canes, a pruning-derived by-product, possess a great amount of bioactive (poly)phenolic compounds belonging to different chemical classes, thus, having a good potential for further valorization. However, in order to properly design valorization strategies, the precise chemical composition of this material has to be known. Up to now, this chemical(More)
In this work, a new alternative for the downstream processing and valorization of black chokeberry pomace (Aronia melanocarpa) which could be potentially coupled to a biorefinery process is proposed. This alternative is based on the application of pressurized liquid extraction (PLE) to the residue obtained after the supercritical fluid extraction of the(More)
1 Characterization of grape seed procyanidins by comprehensive two-dimensional hydrophilic interaction x reversed phase liquid chromatography coupled to diode array detection and tandem mass spectrometry (HILICxRPLC-DAD-MS/MS). Lidia Montero, Miguel Herrero*, Marin Prodanov, Elena Ibáñez, Alejandro Cifuentes Laboratory of Foodomics, Institute of Food(More)