Lichuan Zhang

Learn More
Fibroblasts, the major cell type in tumor stroma, are essential for tumor growth and survival, and represent an important therapeutic target for cancers. Here we presented a microfluidic co-culture device in which the three-dimensional (3D) matrix was employed to reconstruct an in vivo-like fibroblast-tumor cell microenvironment for investigation of the(More)
This study aimed to determine the relationship between the endogenous levels of P-glycoprotein (P-gp), multidrug resistance-associated protein (MRP), lung resistance-related protein (LRP), glutathione-s-transferase-π (GST‑π) and topoisomerase IIα (TopoIIα) and intrinsic drug resistance in four human lung cancer cell lines, SK-MES-1, SPCA-1, NCI-H-460 and(More)
Microchip-based systems have been developed rapidly due to their desirable advantages over conventional platforms. Higher level system integration and complex microdevices are emerging to satisfy the demand for high-throughput and large-scale applications. However, most of the devices need to be fabricated with complicated microvalves and micropumps, which,(More)
The upregulation of GRP78 and GRP94 under the induction of A23187 and its function in drug resistance to etoposide (VP-16) was investigated in human lung cancer cell line SK-MES-1. The expression of GRP78 and GRP94 induced by A23187 at different concentrations was analyzed by RT-PCR and Western blotting. Cell survival to VP-16 was determined using a(More)
INTRODUCTION Heavy cigarette smoking-related chronic obstructive pulmonary disease is an independent risk factor for lung squamous carcinoma. However, the mechanisms underlying the malignant transformation of bronchial epithelial cells are unclear. METHODS In our study, human tumor-adjacent bronchial epithelial cells were obtained from 10 cases with(More)
In this paper, we propose a cooperative localization algorithm based on Extended Kalman Filter (EKF) for multiple underwater vehicles. Research on cooperative localization of multiple underwater vehicles is an important topic to solve the navigation problem in long range and deep sea. We investigate the improvement in navigation accuracy. In the cooperative(More)
Recently there has been a more focus on the development of an efficient technique for detection of circulating tumor cells (CTCs), due to their significance in prognosis and therapy of metastatic cancer. However, it remains a challenge because of the low count of CTCs in the blood. Herein, a rapid and high-sensitivity approach for CTCs detection using an(More)
BACKGROUND AND OBJECTIVE Hsp90 is a major molecular chaperone, which overexpression is involved in oncogenesis, development and drug resistance in many human cancers. The aim of this study is to investigate the relationship between the GGA-induced overexpression of Hsp90 and chemoresistance to Cisplatin in SPCA-1 and H446 cell line. METHODS The protein(More)
A ballistic missile is one of the main targets of space target defense and often has an established trajectory. As the target, we are only interested in its trajectory. The intercepting missile, as a defense body, controls itself toward the target according to the target state observed information and the control strategies in real time. So, the kinematic(More)
BACKGROUND GRP78, a member of GRPs, plays a critical role in chemotherapy resistance in some cancers. To investigate the relationship between the expression of GRP78 and resistance to anti-cancer drug VP-16 in vitro in lung adenocarcinoma SPCA-1 cell line. METHODS SPCA-1 cells were divided into three groups: BAPTA-AM-treated group, A23187-treated group(More)