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To retrospectively compare contrast-enhanced multislice computed tomography (MSCT) and ultrasound (US) imaging for the assessment of cystic renal masses in children using the Bosniak classification system. Twenty-two consecutive patients (age 1 month to 5.2 years, mean 2.4 years) with 24 cystic renal masses (7 benign, 17 malignant) pathologically confirmed(More)
A novel magneto-controlled electrochemical DNA biosensor is designed for the ultrasensitive detection of lead coupling a lead-specific DNAzyme with DNA-based hybridization chain reaction (HCR). To construct such a magnetic lead sensor, DNAzyme-based molecular beacons, selective to cleavage in the presence of Pb(2+), are initially immobilized onto magnetic(More)
Methods based on metal nanotags have been developed for metallobioassay of nucleic acids, but most involve complicated labeling or stripping procedures and are unsuitable for routine use. Herein, we report the proof-of-concept of a novel and label-free metallobioassay for ultrasensitive electronic determination of human immunodeficiency virus (HIV)-related(More)
PURPOSE To investigate the clinical features of pediatric Xp11.2 translocation renal cell carcinoma (RCC). METHODS A retrospective review of 22 cases over 35 years. RESULTS Xp11.2 translocation RCCs were identified in 13 boys and 9 girls with a median age of 10.5 years (range: 2.5-16 years). RCC presented with hematuria in 17, abdominal mass in 1,(More)
A simple, sensitive and reusable electrochemical sensor was designed for determination of mercury (II) (Hg(2+)) by coupling target-induced conformational switch of DNA hairpins with thymine-Hg(2+)-thymine (T-Hg(2+)-T) coordination chemistry. The hairpin probe consisted of a stem of 6 base pairs enclosing a 14 nucleotide (nt) loop and an additional 12 nt(More)
A new and disposable electrochemical immunosensor was designed for detection of alpha-fetoprotein (AFP), as a model analyte, with sensitivity enhancement based on enzyme-catalyzed silver deposition onto irregular-shaped gold nanoparticles (ISGNPs). The assay was carried out with a sandwich-type immunoassay protocol by using ISGNP-labeled anti-AFP antibodies(More)
This works reports a new signal-on amplification strategy for sensitive electronic detection of nucleic acid based on the isothermal circular strand-displacement polymerization (ICSDP) reaction. The assay mainly involves a hybridization of ferrocene-labeled hairpin DNA with blocker DNA, a strand-displacement process with target DNA, and an ICSDP-based(More)
OBJECTIVE To study the clinicopathological and immunohistochemical features, histogenesis and prognosis of pleuropulmonary blastoma (PPB) in children. METHODS PPB specimens from 16 pediatric cases with an age ranging from 1 year and 7 months to 5 years and 3 months (mean age of 3 years) were retrieved and analyzed by routine histological,(More)
Squaric acid, a 2-dimensional planar structure of squarate C4O4 units linked by protons in a layered sheet, was utilized for the first time as a catalytic substrate for ultrasensitive electronic determination of low-abundance proteins by coupling a target-induced electrocatalytic reaction with the in situ cycling signal amplification strategy.
Many efforts have been made to address involvement of the insulin-like growth-factor (IGF) pathway in rhabdomyosarcoma (RMS) pathogenesis, but the actual role of IGF in RMS is still controversial. OBJECTIVE To investigate the implications of IGF2, IGFBP2 and p63 in RMS, and further explored their potential interaction. DESIGN A total of 114 specimens of(More)