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Protein lysine acetylation is a reversible and dynamic post-translational modification. It plays an important role in regulating diverse cellular processes including chromatin dynamic, metabolic pathways, and transcription in both prokaryotes and eukaryotes. Although studies of lysine acetylome in plants have been reported, the throughput was not high(More)
Calbindin-D28K (Calbindin) is a member of the superfamily of calcium-binding proteins that is implicated in the regulation of intracellular calcium. In the adult mammalian brain, calbindin was thought to be present only in neurones, where it is believed to serve a neuroprotective role. We now report the expression of calbindin after ischaemia in reactive(More)
OBJECTIVE Apoptosis of neuronal cells plays a key role in many developmental and pathological processes of the central nervous system. Deoxyribonucleic acid (DNA) of cells undergoing apoptosis is cleaved by an endonuclease into oligonucleosoma-sized fragments. These fragments can be labeled using in situ terminal deoxynucleotidyl transferase so that the(More)
Immunoreactivity of neuronal and glial marker proteins of reactive astrocytes around the electrically damaged pyramidal layer and stratum radiatum of the hippocampal CA1 region and corpus callosum was chronologically studied in electrically lesioned rat brains. A monoclonal antibody against calbindin-D28 k (CD28-Ab) and a polyclonal antibody against glial(More)
With the use of double immunofluorescence, we have examined the distribution of oxytocin-containing neurons that express substance P receptor (SPR) in the hypothalamus of C57 mice. The distribution of oxytocin-like immunoreactive neurons overlapped with that of SPR-like immunoreactive neurons in the paraventricular nucleus and supraoptic nucleus of the(More)
Mice that express transgenes for human papillomavirus type 16 under a keratin 14 promoter (K14-HPV16 mice) develop cervical cancer when they are given 17␤-estradiol chronically. We asked whether the antiestro-genic phytochemical indole-3-carbinol (I3C), found in cruciferous vegetables , administered at physiological doses, would prevent the cervical-vaginal(More)
BACKGROUND To investigate the regulation of K17 expression by the pro-inflammatory cytokine IL-22 in keratinocytes and its important role in our previously hypothesized "K17/T cell/cytokine autoimmune loop" in psoriasis. MATERIALS AND METHODS K17 expression was examined in the IL-22-treated keratinocytes by real-time quantitative PCR, ELISA, Western blot(More)
The pathogenesis of bullous pemphigoid (BP) is characterized by the T cell-dependent production of autoantibodies. Recent studies have indicated that follicular T helper cells (Tfh), the key modulator of B cell activation and autoantibody production, are critical in the development of several autoimmune diseases. Tfh cells perform their functions via IL-21,(More)
Diosgenin is a steroid derived from cholesterol in plants and used as a typical initial intermediate for synthesis of numerous steroidal drugs in the world. Commercially, this compound is extracted mainly from the rhizomes or tubers of some Dioscorea species. Squalene synthase (SQS: EC 2.5.1.21) catalyzes the condensation of two molecules of farnesyl(More)
Pseudoxanthoma elasticum (PXE), an autosomal recessive disorder characterized by ectopic mineralization, is caused by mutations in the ABCC6 gene. We examined clinically 29 Chinese PXE patients from unrelated families, so far the largest cohort of Asian PXE patients. In a subset of 22 patients, we sequenced ABCC6 and another candidate gene, ENPP1, and(More)