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OBJECTIVE To determine the contents of tetramethylpyrazine (TMP) and ferulic acid in Ligusticum chuanxiong from different producing areas and seasons. METHODS The contents of TMP and ferulic acid were determined by HPLC, and then analyzed by Chromatographic Fingerprints. RESULTS The contents of TMP and ferulic acid from different seasons were obviously(More)
A fusion gene was constructed in a plasmid in which the coding regions of human GM_CSF and LIF cDNAs were connected by a synthetic linker sequence encoding a short peptide G-S-G-G-S through DNA recombinant techniques. It was then subcloned into the pBV220 expression vector, and expressed in E. coli after transformation and temperature induction. The(More)
MUC1 mucin is a high molecular weight, type I transmembrane glycoprotein. High and aberrant expression of MUC1 is observed in various types of tumors, which make it an ideal target for tumor biotherapy as well as a biomarker for tumor diagnosis and prognosis. MUC1/Y is an isoform of MUC1 generated by alternative splicing. Specific expression of MUC1/Y in(More)
OBJECTIVE To study on invasion and metastasis-associated genes of lung cancer related with NM23-H1 gene. METHODS Human gene expression chip based on the subtracted cDNA libraries was constructed. After microarray hybridization, clones sequencing, sequence homology search, the information of differently expressed genes in human large cell lung cancer cell(More)
BACKGROUND & OBJECTIVE Ovarian cancer is the leading cause of death among gynecological malignancies. Up to now, little is known about specific tumor-suppressor genes or oncogenes involved in the ovarian cancer genesis. Thus isolation of new candidate genes and characterization of their role in ovarian cancer genesis will be helpful for understanding the(More)
This study was aimed to compare the differential expressions of calcineurin (PP2B, PP3) in the mouse Pre-B cell lines (S9) and the tumor cell lines (S4C2) derived from pre-B lymphocytes, and to clarify its possible mechanism involving in the leukemia cell apoptosis. The quantitative real-time PCR was used to detect the differential expressions of(More)
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