Li-shen Yu

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Polymorphisms of the cytochrome P450 2D6 (CYP2D6) gene play a major role in pharmacokinetic variability in human, while CYP2D6*10 is an important subtype in Asian people. In this study, the co-expression enzyme of human recombinant CYPOR, CYPb5 and CYP2D6.1 or CYP2D6.10 with the Bac-to-Bac system in baculovirus-infected insect cells was used to study the(More)
Early stationary phase cells of Listeria monocytogenes (Scott A) were examined to determine the effect of heat-shock on the production of listeriolysin O (LLO) during and after resuscitation at 37 degrees C. Cells were subjected to a heat-shock at 48 degrees C for 1 h. Intracellular and extracellular proteins of the heat-shocked cells were assayed for LLO(More)
OBJECTIVE To demonstrate the tissue specific expression of five rat genes related to centrifuge training and the relationship between expression change and duration of training. METHOD mRNA was extracted from hearts, brains, kidneys, lungs and intestines of rats after different durations of training. Five genes obtained by SSH from centrifuge-trained rats(More)
Ginkgolic acids have been shown to possess allergenic as well as genotoxic and cytotoxic properties. The question arises whether the metabolism of ginkgolic acids in the liver could decrease or increase their toxicity. In this study, the in vitro metabolism of ginkgolic acid (15:1, GA), one component of ginkgo acids, was investigated as a model compound in(More)
The oral uptake of zolmitriptan, a novel and highly selectively 5-HT 1B/1D receptor agonist, was evaluated in the human epithelial cell line caco-2 that possesses intestinal enterocyte-like properties when cultured in vitro. The study demonstrated that zolmitriptan uptake significantly depended upon the extracelluar temperature and pH in the Caco-2 cell.(More)
Objective. To observe the subjects' perception of orientation following certain head movements or change of simulator cab attitude in hypergravity (HG), and assess the feasibility of simulating G-excess illusion on the ground by a centrifuge-like Spatial Disorientation (SD) simulator. Method. 1.6 G force field was generated by planetary rotation of the(More)
The aim of the present study was to develop the recombinant insect cell-expressed protein as an in vitro model for inhibitors screening for human cytochrome P450 2C19 (CYP2C19), and to use the model to investigate the inhibition effect of three phytochemicals on CYP2C19 in vitro. Omeprazole was applied as the probe substrate. The estimated inhibitory(More)
OBJECTIVE To investigate the development of vestibular system function in 7 to 14 years old normal boys and the ADHD boys; METHODS The present study involved two groups of subjects: 148 ADHD boys (age range 7 years to 14 years) diagnosed with DSM-IV diagnostic criteria and 148 normal boys with the same age. Each group was divided into 4 clusters according(More)
OBJECTIVE To examine the time course and clinical relevance of changes in prostaglandin E2 (PGE2) to pain and outcome in surgical drainage, and circulating blood after functional endoscopic sinus surgery (FESS). METHODS Thirty-seven patients scheduled to undergo FESS were randomized to receive celecoxib 400 mg(n=16) one hour before anesthesia induction(More)
OBJECTIVE To explore if high G exposure can cause vestibular disorder in guinea pig and the possibility of preventing it by preconditioning with exposure to a low hypergravity environment before high G stimulus. METHOD A total of 86 guinea pigs were divided into 4 groups: 1) 28 were treated with a stimulus of +10 Gy for 5 min (+10 Gy group); 2) 28 were(More)