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BACKGROUND The sensitivity and selectivity of traditional methods limits ultramicro detection of proteins. Bio-barcode amplification detection methods based on nanotechnology enables ultramicro detection of protein. However, bio-barcode amplification detection depends on the oligonucleotides being fixed on a glass chip. It also requires specialized(More)
OBJECTIVE To identify the candidate genes within the putative susceptibility locus for systemic lupus erythematosus (SLE) at 12p12.3-13.2. METHODS KLRC1 was selected as the candidate gene according to the results of previous gene chip studies. TaqMan real-time quantitative PCR was performed for detecting KLRC1 mRNA expression in 55 SLE patients and 30(More)
AIM To investigate the effect of transplantation of bone marrow-derived mesenchymal stem cells (MSCs), or co-transplantation of islet and MSCs, on maturation and function of bone marrow-derived dendritic cells (DCs) in recipient mice. METHODS Bone marrow MSCs were isolated from BALB/c mice and co-cultured with bone marrow mononuclear cells (BMCs) from(More)
Group 1 rabbits received cholesterol (CS) in a dose of 0.5 g/kg/day, per os, during 4, 8 and 12 weeks that resulted in a significant increase of the total CS level in the blood serum of the total lipids (TL), phospholipids and beta-lipoproteins (3-10 times). Combined administration of CS and 40% eicosapentaenoic acid (EPA), 0.6 ml/kg, to group 2 rabbits led(More)
OBJECTIVE to investigate the effect of gene silencing of integrin β1 on the proliferation and secretory function of airway smooth muscle cells (ASMC) in asthmatic mice. METHODS integrin β1 gene was silenced by using RNAi technology in the fifth generation ASMC of normal and asthmatic mice. The integrin β1 mRNA expression and integrin β1 protein were(More)
AIM To analyze the surface markers on peripheral blood dendritic cells (DCs) in SLE patients and explore the relationship between the DCs and pathogenesis of SLE. METHODS The peripheral blood monouclear cells (PBMCs) were separated by density gradient centrifugation. After culture of 3 hours in tissue culture flask, the suspended cells were removed and(More)
Glucagon and concanavalin A were administered into cell cultures of mice peritoneal macrophages and human intima aorta simultaneously with atherogenous blood serum, obtained from patients with ischemic heart disease, or with acetylated and native low density lipo-proteins. Their effect was dissimilar: glucagon decreased accumulation of intracellular(More)
Consumption of 15 g of 40% eicosapentaenic acid within 3 days resulted in 3-fold increase of its content in blood serum of healthy persons and of patients with heart ischemic disease, whereas concentration of arachidonic acid was unaltered. Administration of blood serum containing increased content of eicosapentaenic acid into cell culture of human aorta(More)
AIM To determine the involvement of NO signal pathway in the development of hyperalgesia induced by activation of protein kinase C (PKC ), nociceptive responses and nitric oxide synthase(NOS) expression and nitric oxide (NO) content in the spinal cord were observed after administration of Phorbol 12-Myristate-Acetate (PMA), a PKC agonist, in rats. METHODS(More)