Li-jun Qin

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Two expression cassettes, the Nicotiana tabacum Ubi.U 4 promoter-driven tobacco mosaic virus (TMV)-resistant gene NrCN element and Arabidopsis thaliana senescence-expressive gene SAG 12 promoter-driven LoxP/FRT site-specific recombinase gene FLP expression fragment, were both introduced into the TMV-sensitive tobacco variety K326 via Agrobacterium(More)
Inositol is a cyclic polyol that is involved in various physiological processes, including signal transduction and stress adaptation in plants. l-myo-inositol monophosphatase (IMPase) is one of the metal-dependent phosphatase family members and catalyzes the last reaction step of biosynthesis of inositol. Although increased IMPase activity induced by(More)
The CYP82E4v1 gene was identified to encode nicotine demethylase, which catalyzed the conversion of nicotine to nornicotine. In this study, we constructed CYP82E4v1-RNAi vector and genetically transformed tobacco variety K326. The determination results of nicotine and nornicotine content via HPLC demonstrated that there was significant increase of nicotine(More)
A method was proposed for the determination of trace copper and lead in beer with flame atomic absorption spectrometry after preconcentration of copper and lead by rapid coprecipitation technique with 8-oxyquinoline-Mg(II) using manganese as an internal standard at pH 9. The standard addition recovery of lead is between 97.6%-103.0%. The detection limit is(More)
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