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The objective of this study to design a delivery system resistant to the gastrointestinal environment for oral vaccine against porcine rotavirus. Lactococcus lactis NZ9000 was transformed with segments of vP4 of the porcine rotavirus inserted into the pNZ8112 surface-expression vector, and a recombinant L. lactis expressing VP4 protein was constructed. An(More)
—This paper investigates a high-efficiency clamped-voltage dc–dc converter with reduced reverse-recovery current and switch-voltage stress. In the circuit topology, it is designed by way of the combination of inductor and transformer to increase the corresponding voltage gain. Moreover, one additional inductor provides the reverse-current path of the(More)
OBJECTIVE This study aimed to investigate neuronal apoptosis and expression of apoptosis related proteins (Fas, Caspase-3 and Bcl-2) in the brain of rates with morphine addiction. METHODS A total of 48 adult male Sprague-Dawley rats weighing 190-210 g were randomly divided into 3 groups (n=16 per group): morphine addiction group, morphine abstinence group(More)
BACKGROUND AND OBJECTIVES Postoperative analgesia is crucial for early functional excise after total knee arthroplasty. To investigate the clinical efficacy of ultrasound and nerve stimulator guided continuous femoral nerve block analgesia after total knee arthroplasty. METHODS 46 patients with ASA grade I-III who underwent total knee arthroplasty(More)
The aim of the present study was to determine the potential long-term metabolic effects of early nutritional programming on carbohydrate utilisation in adult zebrafish (Danio rerio). High-carbohydrate diets were fed to fish during four ontogenetic stages: from the first-feeding stage to the end of the yolk-sac larval stage; from the first-feeding stage to 2(More)
Electromyograph (EMG) features have the properties of large variations and nonstationarity. An important issue in the classification of EMG is the classifier design. The major goal of this paper is to develop a classifier for the classification of eight kinds of prehensile postures to achieve high classification rate and reduce the online learning time. The(More)
Polybia-MPI (MPI), a short cationic α-helical antimicrobial peptide, exhibited excellent anticancer activity and selectivity in vitro in our previous studies. To improve its in vivo application, we synthesized an analog (MPI-1) of MPI by replacing the C terminal amide -[CO-NH(2)] with thioamide -ψ[CS-NH(2)]. Although there is just one atom difference, the(More)
Tracking objects that undergo abrupt appearance changes and heavy occlusions is a challenging problem which conventional tracking methods can barely handle. To address the problem, we propose an online structure learning algorithm that contains three layers: an object is represented by a mixture of online structure models (OSMs) which are learnt from(More)