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BACKGROUND The value of oocyte cryopreservation remains controversial. Two major problems exist: poor survival and injury to the oocyte meiotic spindle after freezing and thawing. METHODS For slow oocyte cryopreservation, we used 1.5 mol/l 1,2-propanediol and 0.3 mol/l sucrose. We waited 3 h after thawing for possible recovery of the meiotic spindles(More)
STUDY QUESTION What is the value of a new strategy for preimplantation genetic diagnosis (PGD) of monogenic diseases: blastocyst biopsy, cryopreservation and thawed embryo transfer? SUMMARY ANSWER This strategy is highly effective for PGD of monogenic diseases and merits wide use. WHAT IS KNOWN ALREADY PGD of monogenic diseases is conventionally(More)
OBJECTIVE To ascertain effects of thermal change, size of blastocoele, artificial shrinkage, and cytoskeletal stabilizer on survival of blastocysts from vitrification. DESIGN In vitro and in vivo study. SETTING University infertility clinic and academic research laboratory. ANIMAL(S) Female mice of outbred ICR strain, aged 6 to 8 weeks. (More)
OBJECTIVE Preimplantation genetic diagnosis (PGD) offers an alternative for women to carry an unaffected fetus risk of hereditary diseases. Trophectoderm biopsy may provide more cells for accurate diagnosis. However, the time allowed for transportation of the specimens to the laboratory and performance of molecular diagnosis is limited. We designed a PGD(More)
BACKGROUND/PURPOSE Patients with chromosomal translocation are highly vulnerable to produce unbalanced gametes that result in recurrent miscarriages, affected offspring, or infertility. Preimplantation genetic diagnosis (PGD) with blastomere biopsy and fluorescent in-situ hybridization (FISH) has been used to select normal/balanced embryos for transfer.(More)
UNLABELLED Diabetes has been consistently linked to many forms of cancers, such as liver, colorectal, pancreatic, and breast cancer, however, the role of diabetes in outcome among cancer patients remains unclear. In this study, we retrospectively reviewed electronic medical records of 342 inpatients newly diagnosed with NSCLC referred by a teaching hospital(More)
OBJECTIVE To determine the efficiency of embryo scoring systems for multiple pregnancy in women undergoing IVF procedures. DESIGN Retrospective record analysis. SETTING University hospital, tertiary medical center. PATIENT(S) Three hundred one patients undergoing controlled ovarian stimulation, IVF/intracytoplasmic sperm injection (ICSI), and day 3(More)