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Sensitization of the pain-transducing ion channel TRPV1 underlies thermal hyperalgesia by proalgesic agents such as nerve growth factor (NGF). The currently accepted model is that the NGF-mediated increase in TRPV1 function during hyperalgesia utilizes activation of phospholipase C (PLC) to cleave PIP2, proposed to tonically inhibit TRPV1. In this study, we(More)
Once thought of as simply an oily barrier that maintains cellular integrity, lipids are now known to play an active role in a large variety of cellular processes. Phosphoinositides are of particular interest because of their remarkable ability to affect many signaling pathways. Ion channels and transporters are an important target of phosphoinositide(More)
Although a large number of ion channels are now believed to be regulated by phosphoinositides, particularly phosphoinositide 4,5-bisphosphate (PIP2), the mechanisms involved in phosphoinositide regulation are unclear. For the TRP superfamily of ion channels, the role and mechanism of PIP2 modulation has been especially difficult to resolve. Outstanding(More)
We developed the LigAmp assay for sensitive detection and accurate quantification of viruses and cells with single-base mutations. In LigAmp, two oligonucleotides are hybridized adjacently to a DNA template. One oligonucleotide matches the target sequence and contains a probe sequence. If the target sequence is present, the oligonucleotides are ligated(More)
BACKGROUND, AIM, AND SCOPE Composting is an effective treatment process to realize sludge land application. However, nitrogen loss could result in the reduction of nutrient value of the compost products and the stabilization effect of composting on heavy metal concentration and mobility in sludge has been shown to be very limited. MATERIALS AND METHODS(More)
Mutator phenotypes are involved in the carcinogenesis of some cancers, e.g., defects in mismatch repair produce a mutator phenotype that drives carcinogenesis and causes microsatellite instability in hereditary nonpolyposis colon cancers and some sporadic colorectal cancers (CRC). Less understood, however, is the potential role of mutator phenotypes in(More)
Heavy metal pollution of soil is a significant environmental problem with a negative potential impact on human health and agriculture. Rhizosphere, as an important interface of soil and plants, plays a significant role in phytoremediation of contaminated soil by heavy metals, in which, microbial populations are known to affect heavy metal mobility and(More)
OBJECTIVE To investigate the heavy metals (HMs) and polycyclic aromatic hydrocarbons (PAHs) in sludge of twelve wastewater treatment plants (WWTPs) in Zhejiang province of China, and to assess their potential for land application. METHODS Sludge was collected from 12 WWTPs within the province. GC-MS and AAS were used to measure PAHs and HMs contents in(More)
Vector wavelet transforms for vector-valued fields can be implemented directly from multiwavelets; however, existing multiwavelets offer surprisingly poor performance for transforms in vector-valued signalprocessing applications. In this paper, the reason for this performance failure is identified, and a remedy is proposed. A multiwavelet design criterion,(More)
Cyclic nucleotide-gated (CNG) channels are nonselective cation channels that are activated by the direct binding of the cyclic nucleotides cAMP and cGMP. The region linking the last membrane-spanning region (S6) to the cyclic nucleotide binding domain in the COOH terminus, termed the C-linker, has been shown to play an important role in coupling cyclic(More)