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An electrochemical hybridization biosensor based on peptide nucleic acid (PNA) probe is presented. PNA were attached covalently onto a quinone-based electroactive polymer. Changes in flexibility of the PNA probe strand upon hybridization generates electrochemical changes at the polymer-solution interface. A reagentless and direct electrochemical detection(More)
We have constructed a reagentless lactate sensor using lactate oxidase (LOD) covalently attached to an electropolymerized copolymer film, poly(5-hydroxy-1,4-naphthoquinone-co-5-hydroxy-3-thioacetic acid-1,4-naphthoquinone), poly(JUG-co-JUGA). Around 10(-12)Mcm(-2) of covalently bound enzymes are immobilized on these films. In aerated medium, the(More)
Our previous studies have shown that the baicalin could blocked infection of chlamydia trachomatis (C. trachomatis)-infected cells in vitro. Toll-like receptor 2 and 4 (TLR2/4) and the downstream nuclear factor-κB (NF-κB) signaling pathway, which mediate the inflammatory reaction, are involved in the pathophysiological processes of inflammation. In this(More)
In this paper, we present a new improved Active Shape Model (ASM) for facial feature points extraction. ASM performance is often influenced by some factors such as initial position, illumination, pose, etc, which frequently lead to the local minima in optimization. The original ASM has two sub-models: global shape model and local texture model, we proposed(More)
ERP systems have become the system of choice for the majority of publicly traded companies and have radically changed the way accounting information is processed, prepared, audited, and disseminated. In this study, we examine whether ERP system implementations have affected the extent to which firms manage earnings amounts and release dates. We find, for a(More)
PURPOSE Low temperature (hypothermia) during irradiation of cells has been reported to have a radioprotective effect. The mechanisms are not fully understood. This study further investigates the possible mechanisms behind hypothermia-mediated radioprotection. MATERIALS AND METHODS Human lymphoblastoid TK6 cells were incubated for 20 min at 0.8 or 37°C and(More)
A system device using multifunctional conjugated copolymer poly(5-hydroxy-1,4-naphthoquinone-co-hydroxy-2-thioacetic acid-1,4-naphthoquinone) acting both as immobilizing and transducing element for reagentless immunosensor has been constructed. Its functionality was evaluated in an antigen-antibody interaction model using ovalbumin-anti-ovalbumin. It was(More)
Allozyme analysis, microsatellite primer PCR (SSRP-PCR), and amplified fragment length polymorphism (AFLP) techniques were used to assess genetic diversity and population structure of the Chinese oriental migratory locust, Locusta migratoria manilensis. A total of 299 PCR markers (67 SSRPs and 232 AFLPs) were detected in eight populations, of which 98.7%(More)
The objective of this paper is to propose a new system for fault diagnosis of train bearings using PCA and ACO. On the base of the analysis of time and frequency domain statistical features extracted from the vibration signals collected from the bearings, twenty features which were the most sensitive to different working states were chosen as the object of(More)
This paper presents techniques developed for lane following and obstacle detection capabilities for autonomous vehicles. The proposed system is based on a single 120 degrees fish eye camera and a 180 degrees scanning LIDAR sensor. For the lane detection a modified lane marking technique was used representing the position of the lines making the lane with(More)