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Functional analysis of the proteome is an essential part of genomic research. To facilitate different proteomic approaches, a MORF (moveable ORF) library of 5854 yeast expression plasmids was constructed, each expressing a sequence-verified ORF as a C-terminal ORF fusion protein, under regulated control. Analysis of 5573 MORFs demonstrates that nearly all(More)
We synthesized reversible terminators with tethered inhibitors for next-generation sequencing. These were efficiently incorporated with high fidelity while preventing incorporation of additional nucleotides, and we used them to sequence canine bacterial artificial chromosomes in a single-molecule system that provided even coverage for over 99% of the region(More)
To further understand the roles of protein glycosylation in eukaryotes, we globally identified glycan-containing proteins in yeast. A fluorescent lectin binding assay was developed and used to screen protein microarrays containing over 5000 proteins purified from yeast. A total of 534 yeast proteins were identified that bound either Concanavalin A (ConA) or(More)
We recently introduced a method to tether intact phospholipid vesicles onto a fluid supported lipid bilayer using DNA hybridization (Yoshina-Ishii, C.; Miller, G. P.; Kraft, M. L; Kool, E. T.; Boxer, S. G. J. Am. Chem. Soc. 2005, 127, 1356-1357). Once tethered, the vesicles can diffuse in two dimensions parallel to the supported membrane surface. The(More)
Over the past 5 years, protein-chip technology has emerged as a useful tool for the study of many kinds of protein interactions and biochemical activities. The construction of Saccharomyces cerevisiae whole-proteome arrays has enabled further studies of such interactions in a proteome-wide context. Here, we explore some of the recent advances that have been(More)
*Fax: (+1) 858-784-7472, floyd@scripps.edu, Homepage: http://www.scripps.edu/chem/romesberg. Supporting information for this article is available on the WWW under http://www.angewandte.org or from the author. NIH Public Access Author Manuscript Angew Chem Int Ed Engl. Author manuscript; available in PMC 2011 August 9. Published in final edited form as:(More)
We describe a method for making and erasing barriers to the lateral diffusion of membrane components in fluid lipid bilayers supported on glass substrates. When a bilayer is mechanically partitioned by scratching the membrane-coated surface at basic pH, barriers to lateral diffusion are formed which prevent mixing between the regions separated by the(More)
tion in affected monolayer regions, as the enhanced aliquid-likeo character of debonded tails would give rise to an enhanced viscous drag opposing the motion of the tip. [10] Attempts to remove from the surface monolayer molecules (following inscription of patterns in the non-destructive patterning regime) by solvent treatments with sonication and(More)
Two methods for patterning surfaces with supported lipid bilayers and immobilized protein are described. First, proteins are used to fabricate corrals for supported lipid bilayers. Poly(dimethylsiloxane) stamps are used to deposit arbitrarily shaped patterns of thin layers of immobilized protein onto glass surfaces. This is followed by vesicle fusion into(More)