Leslie E F Moffat

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virus (RSV)–PP1, CMV-PP2A, CMV-PPIV, or CMV-PPV expression plasmid was cotransfected along with the reporter plasmid. Cells were treated with 1 nM Shh and assayed for reporter gene activity. 25. An internal control containing a 763-bp insert into the Sph I site of the Islet1 cDNA was transcribed in vitro to prepare sense-oriented RNA. One nanogram of this(More)
BACKGROUND The most frequent site of metastases from prostate cancer is bone. Bisphosphonates reduce excessive bone turnover while preserving bone structure and mineralization in patients with other tumor types. We conducted a double-blind, placebo-controlled, randomized trial to determine whether the first-generation bisphosphonate sodium clodronate could(More)
There is increasing evidence that essential fatty acids (EFA) may have a role to play in the aetiology of some types of cancer although their precise mode of action is unknown. Differences in the metabolism of EFA between patients with benign or malignant prostatic disease may help to elucidate their role in the latter. We have, therefore, measured the(More)
We have examined the composition of the essential fatty acids in the plasma phospholipid fractions of 98 patients with histologically proven bladder cancer. These patients were attending hospital for regular follow-up by check cystoscopy. Patients were divided into two groups, depending on the cystoscopic findings, of either active (tumour recurrence seen)(More)
BACKGROUND Germline mutations within DNA-repair genes are implicated in susceptibility to multiple forms of cancer. For prostate cancer (PrCa), rare mutations in BRCA2 and BRCA1 give rise to moderately elevated risk, whereas two of B100 common, low-penetrance PrCa susceptibility variants identified so far by genome-wide association studies implicate RAD51B(More)
transfection cocktail. Forty-eight hours after transfec-tion, cell extracts were prepared and assayed for 13-ga-lactosidase activity (26). Appropriately normalized amounts of cell extract were used for CAT assays (14). After autoradiographic exposure, the thin-layer chro-motography (TLC) plates were scanned, and percent conversion values were calculated.(More)
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