Leonid I Marinin

Learn More
It has been shown that antianthrax immunity induced by the novel vaccine proposed has not only antitoxic, but also antispore character. The whole complex of antigens, namely surface spore antigens, surface antigens of cell wall and toxin components is required for the induction of strong and stable immunity against anthrax. The STI-1 vaccine strain with(More)
The Gram-negative bacterium Lysobacter sp. XL1 secretes lytic enzymes (L1-L5) into the culture medium. Enzyme L5 is the most recently found extracellular lytic enzyme of this bacterium. The paper presents the results of the isolation and characterization of some properties of this enzyme. Thus, enzyme L5 of Lysobacter sp. XL1 is a lytic serine protease.(More)
In vivo and in vitro efficacy of tetracyclines was studied with respect to anthracic infection induced by a tetracycline-resistant resistant strain containing plasmid pBC16. The plasmid-containing strain was resistant to tetracycline, doxycycline and minocycline, the MICs exceeding those for the initial strain 500, 640 and 80 times, respectively. There was(More)
The cereolysin AB genes from Bacillus cereus VKM-B164 have been expressed in Bacillus anthracis strains: virulent H-7 (PXO1, PXO2), vaccine STI-1 (PXO1), 221 (without its own plasmids). Expression was achieved by cloning the genes in a high copy number plasmid pE194. This construct was integrated with host genomes in amplified form. Gold hamsters were(More)
Formation of spontaneous RifR mutants was detected in the populations of various strains of Bacillus anthracis (STI-1, Sterne and CH-7) at a rate of 10(-8) per 1 CFU. The levels of the rifampicin resistance in the mutants were different, the MIC ranged from 16 to 512 micrograms/ml. The clones of the RifR population of the virulent strain CH-7 were(More)
The results of detection and identification of Bacillus anthracis strains in loop-mediated isothermal DNA amplification (LAMP) reaction performed under optimized conditions with original primers and thermostable DNA polymerase are presented. Reproducible LAMP-based detection of chromosomal and plasmid DNA targets specific for B. anthracis strains has been(More)
The developmental mechanisms of anthrax immunity were studied. Immunization was found to generally generate specific antibodies and lysozyme. Collectively, all the factors are responsible for suppressing the development of spores in the body. This proves the fact that the immunity is directed not only towards the exotoxin of B. anthracis, but it affects(More)
The therapeutic efficacy of enterocin S760, a broad spectrum antimicrobial peptide produced by Enterococcus faecium LWP760 was tested on mice infected with Bacillus anthracis M-71 to induce anthrax (second Tsenkovsky's vaccine). Intraperitoneal four-, two- or one-fold administration of the peptide in a dose of 25 mg/kg for 10 days for prophylactic (1 hour(More)
It has been established that all anthrax bacteria in an infected organism have the same morphology and fine structure typical for Bacillus anthracis cells. Anthrax bacteria under conditions of a macroorganism form a capsule and slime. A massive bacterial capsule 0.1–0.4 μm thick is characteristic for bacteria of highly virulent and virulent B. anthracis(More)
Interaction of the cells of Bacillus anthracis strain CH-7 with benzylpenicillin was studied. The cells of strain CH-7 were shown to contain the penicillinase gene in the repressed state. Spontaneous derepression of the gene at a rate of 10(-8) resulting in the synthesis of penicillinase was observed. Penicillinase was synthesized constitutionally and its(More)