Leonid E Bulychev

Learn More
Antiviral activity of the new chemically synthesized compound NIOCH-14 (a derivative of tricyclodicarboxylic acid) in comparison with ST-246 (the condensed derivative of pyrroledione) was observed in experiments in vitro and in vivo using orthopoxviruses including highly pathogenic ones. After oral administration of NIOCH-14 to outbred ICR mice infected(More)
The experimental data on aerosol challenge of rabbits with Venezuelan equine encephalomyelitis (VEE) virus are presented. Lethal infection of rabbits was followed by a rise in body temperature (greater than 0.5 degrees C) within 1-2 days after challenge. Two waves of lethality in aerosol infection experiments were recorded. Three variants of the infection(More)
Detailed studies of the pathogenesis of certain neuroviral infections allow for a better understanding of the special role of the olfactory neuroepithelial cells in the invasion of viruses into the CNS. Several studies using animal models demonstrated that neurotropic viruses belonging to various families invade the brain via the olfactory tract after(More)
This study demonstrates the possibility of achieving a prophylactic effect by intramuscular injection of Abies sibirica polyprenols for the control of influenza virus infection in mice. One of the five polyprenol preparations tested, preparation N1, which had the lowest hydrophilic-lipophilic balance (8.6), produced a significant protective effect when(More)
Therapeutic and prophylactic effects of immunomodifiers ridostin, reaferon, and polyribonate used alone and in various combinations were assessed in experiments on guinea pigs infected with Venezuelan equine encephalomyelitis (VEE) (strain Trinidad), Marburg (strain Popp), and Ebola (M/C-8 variant of Zaire strain) viruses at doses 5 to 20 respiratory LD50(More)
White mice weighing 14-16 g were intranasally infected with LD50 of influenza virus (A/Aichi/2/68 strain). High levels both of virus and interferon were detected in the lung. Sufficient virus accumulation in the nasal cavity occurred with low interferon induction. At the same time high blood interferon levels corresponded to sporadic low viremia.(More)
The time dependence of interferon production in blood, tissues of the respiratory tract, brain and olfactory tract of mice BALB/c was investigated after administration of the interferon inductor ridostin by various routes. Intraperitoneal injection of ridostin in a dose of 5 mg/kg induced intensive accumulation of interferon in the blood serum with the peak(More)
This research investigates a promising antiviral compound based on polyprenols from Siberian silver fir (Abies sibirica). The physico-chemical characteristics of a preparation developed in aerosol form and an estimation of its protective efficacy against aerosol challenge of laboratory animals are presented. It is shown that (1) by using a simple ultrasonic(More)
Combined application of ridostine with catonic liposomes was shown to essentially enhance the interferon-inducing and antiviral activity of the former in experiments with cell cultures L-929, which is apparently related with an improved efficiency of intracellular delivery of dsRNA. A comparative study demonstrated that ridostine, when combined with(More)
Immunogenicity of recombinant vaccinia virus strain (VR26) expressing Venezuelan equine encephalomyelitis (VEE) virus structural protein genes was studied by oral immunization. Sera of animals immunized with VR26 contained antibodies specific to VEE virus, among which antibodies with virus-neutralizing activity were present. Evaluation of the protective(More)