Leonard E. Gerber

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The effects of all-trans and 13-cis retinoic acid upon serum and liver lipids were investigated in Sprague-Dawley rats. Groups of rats were fed daily with 105, 210 and 315 micrograms/g diet of one of the retinoids for periods of up to 8 days. Other groups were injected intraperitoneally (I.P.) daily with retinoids at levels equivalent to the daily intake of(More)
This study investigated both the possible antiproliferative and proapoptotic effects of, either alpha-tocopherol or gammatocopherol in vitro, upon a human salivary gland cancer cell line (ACC3), derived from an adenoid cystic carcinoma and M1 cells, a non-cancerous human salivary gland cell line. Prior to the use of these cell lines for treatment, they were(More)
The effects of feeding various levels and combinations of retinyl acetate, beta-carotene, or retinoic acid on skin wound healing in rats was investigated. Weanling male Sprague-Dawley rats were fed a vitamin A-free diet for 2 weeks to produce marginal vitamin A status. After a paravertebral incision was made and closed with suture, one of several diets were(More)
Administration of oral vitamin A and the synthetic retinoids to humans and experimental animals has been accompanied by changes in lipid metabolism. these changes include alterations in serum, liver, and skin surface lipids. While the immediate cause(s) of the serum and liver changes remain(s) obscure, the skin surface lipid alterations appear to be(More)
A rapid specific, microdetermination of the major human blood carotenoids by high performance liquid chromatography (HPLC) separation and quantitation at 466 nm is detailed in this paper. Serum retinyl esters can also be quantified utilizing the same separation procedure but detected at 325 nm. One hundred microliters of deproteinated serum were extracted(More)
The effect of beta-carotene on the morphology of NCI-H69 small cell lung cancer cells that had undergone beta-carotene-induced growth reduction (P < 0.05) was examined. The cells were grown at 1 x 10(8) cells/L and were cultured with or without 20 micromol/L beta-carotene. The qualitative electron microscopic observations revealed that beta-carotene-treated(More)
A rapid specific, microdetermination of the major human blood carotenoids by high performance liquid chromatography (HPLC) separation and quantitation at 466 nm is detailed in this paper. Serum retinyl esters can also be quantified utilizing the same separation procedure but detected at 325 nm. One hundred microliters of deproteinated serum were extracted(More)
Delivery of beta-carotene in tetrahydrofuran slowed the growth of NCI-H69 small cell lung cancer cells. Analysis of cells and cellular fractions revealed that beta-carotene-treated cells accumulated beta-carotene as well as some polar metabolites, primarily in the crude nuclei. Cells were grown at 1 x 10(5) cells/ml and treated with 20 microM beta-carotene.(More)