Leonard D Pysh

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In the Arabidopsis root meristem, initial cells undergo asymmetric divisions to generate the cell lineages of the root. The scarecrow mutation results in roots that are missing one cell layer owing to the disruption of an asymmetric division that normally generates cortex and endodermis. Tissue-specific markers indicate that a heterogeneous cell type is(More)
Mutations at the SCARECROW (SCR) locus in Arabidopsis thaliana result in defective radial patterning in the root and shoot. The SCR gene product contains sequences which suggest that it is a transcription factor. A number of Arabidopsis Expressed Sequence Tags (ESTs) have been identified that encode gene products bearing remarkable similarity to SCR(More)
OPAQUE2 (O2) is a regulatory gene that predominantly affects the expression of the 22-kD class of zein storage protein genes at the level of transcription. The O2 gene encodes a polypeptide belonging to the basic domain/leucine zipper (bZIP) class of transcriptional regulatory proteins. Our prior analyses have demonstrated that the O2 protein binds 22-kD(More)
The maize proteins Opaque-2 (O2) and OHP1 are members of the bZIP class of transcriptional regulatory proteins. The genomic organizations of the O2 and OHP1 genes are conserved in terms of the number and placement of introns which suggests a common evolutionary origin. The organization of O2 and OHP1 contrasts with the maize bZIP protein gene OBF1, which(More)
Plant cell shape is determined by the orientation of cellulose microfibrils in the primary cell wall. Consequently, mutations that affect genes encoding the enzymes responsible for the synthesis of cellulose, namely, the cellulose synthase catalytic subunits, can alter cell shape substantially, particularly in the roots of affected plants. The multiple(More)
PREMISE OF THE STUDY Cellulose is the most abundant biomolecule on the planet, yet the mechanism by which it is synthesized by higher plants remains largely unknown. In Arabidopsis thaliana (L.) Heynh, synthesis of cellulose in the primary cell wall requires three different cellulose synthase genes (AtCesA1, AtCesA3, and AtCesA6-related genes [AtCesA2,(More)
OPAQUE2 (02) is a regulatory gene that predominantly affects the expression of the 22-kD class of zein storage protein genes at the leve1 of transcription. The 02 gene encodes a polypeptide belonging to the basic domain/leucine zipper (bZIP) class of transcriptional regulatory proteins. Our prior analyses have demonstrated that the 02 protein binds 22-kD(More)
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