Leo C. McHugh

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Protein identification using mass spectrometry is an indispensable computational tool in the life sciences. A dramatic increase in the use of proteomic strategies to understand the biology of living systems generates an ongoing need for more effective, efficient, and accurate computational methods for protein identification. A wide range of computational(More)
OBJECTIVE To quantify incidence of, and risk factors for, progression to and spontaneous regression of high-grade anal squamous intraepithelial lesions (ASILs). DESIGN Retrospective review of patients at St Vincent's Hospital Anal Cancer Screening Clinic during a period when high-grade ASILs were not routinely treated (2004-2011). METHODS All patients(More)
Protein identification using mass spectrometry is an important tool in many areas of the life sciences, and in proteomics research in particular. Increasing the number of proteins correctly identified is dependent on the ability to include new knowledge about the mass spectrometry fragmentation process, into computational algorithms designed to separate(More)
Gene expression databases contain invaluable information about a range of cell states, but the question "Where is my gene of interest expressed?" remains one of the most difficult to systematically assess when relevant data is derived on different platforms. Barriers to integrating this data include disparities in data formats and scale, a lack of common(More)
The innate immune system of humans and other mammals responds to pathogen-associated molecular patterns (PAMPs) that are conserved across broad classes of infectious agents such as bacteria and viruses. We hypothesized that a blood-based transcriptional signature could be discovered indicating a host systemic response to viral infection. Previous work(More)
Introduction: A hallmark of sepsis is early onset microvascular dysfunction. However, the mechanism responsible for maldistribution of capillary blood flow is not understood. Evidence suggests red blood cells (RBC) can sense local oxygen (O2) conditions and signal the vasculature, via adenosine triphosphate (ATP), to increase capillary flow. We hypothesized(More)
OBJECTIVES SeptiCyte Lab (Immunexpress, Seattle, WA), a molecular signature measuring the relative expression levels of four host messenger RNAs, was developed to discriminate critically ill adults with infection-positive versus infection-negative systemic inflammation. The objective was to assess the performance of Septicyte Lab in critically ill pediatric(More)
Unfortunately, the original version of this supplement [1] contained errors in two of the abstracts; P037 and P127. Please see details below. In P037, the image presented as Figure eight is incorrect. The correct figure is shown below (Fig. 1). In P127, the author list presented is incorrect. The correct author list is as below. R Iqbal; Y Alhamdi; N(More)
BACKGROUND Although anal squamous cell carcinomas (ASCC) are rare in the general community, rates of ASCC among HIV-positive men who have sex with men (MSM) approach those of major cancers in the general community, such as colorectal and lung cancers. Anal cytology and high-resolution anoscopy (HRA) have been proposed as methods for the diagnosis of(More)