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A Potential Antitumor Drug (Arginine Deiminase) Reengineered for Efficient Operation under Physiological Conditions
Arginine deiminase (ADI, EC 3.5.3.6) is a potential antitumor drug for the treatment of arginine‐auxotrophic tumors such as hepatocellular carcinomas (HCCs) and melanomas, and studies on humanExpand
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Directed laccase evolution for improved ionic liquid resistance
In nature, the biodegradation of lignin is a challenging process since lignin is highly cross-linked and poorly water-soluble. Laccases (EC 1.10.3.2, benzenediol: oxygen oxidoreductase) play a keyExpand
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A Competitive Flow Cytometry Screening System for Directed Evolution of Therapeutic Enzyme.
A ligand-mediated eGFP-expression system (LiMEx) was developed as a novel flow cytometry based screening platform that relies on a competitive conversion/binding of arginine between arginineExpand
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Rapid evolution of arginine deiminase for improved anti-tumor activity
  • Ye Ni, Y. Liu, +4 authors Zhihao Sun
  • Biology, Medicine
  • Applied Microbiology and Biotechnology
  • 11 January 2011
Arginine deiminase (ADI), an arginine-degrading enzyme, has been studied as a potential anti-cancer agent for inhibiting arginine-auxotrophic tumors, such as melanomas and hepatocellular carcinomas.Expand
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Multi-step biocatalytic depolymerization of lignin
Lignin is a biomass-derived aromatic polymer that has been identified as a potential renewable source of aromatic chemicals and other valuable compounds. The valorization of lignin, however,Expand
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Protein Engineering of the Antitumor Enzyme PpADI for Improved Thermal Resistance
Arginine deiminase (ADI, EC 3.5.3.6) is a potential antitumor drug for the treatment of arginine‐auxotrophic tumors such as hepatocellular carcinomas (HCCs) and melanomas. Studies in human lymphaticExpand
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Grafting PNIPAAm from β-barrel shaped transmembrane nanopores.
The research on protein-polymer conjugates by grafting from the surface of proteins has gained significant interest in the last decade. While there are many studies with globular proteins, membraneExpand
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Unraveling the effects of amino acid substitutions enhancing lipase resistance to an ionic liquid: a molecular dynamics study.
Understanding of the structural and dynamic properties of enzymes in non-aqueous media (e.g., ionic liquids, ILs) is highly attractive for protein engineers and synthetic biochemists. Despite aExpand
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A hybrid ring-opening metathesis polymerization catalyst based on an engineered variant of the β-barrel protein FhuA.
A β-barrel protein hybrid catalyst was prepared by covalently anchoring a Grubbs-Hoveyda type olefin metathesis catalyst at a single accessible cysteine amino acid in the barrel interior of a variantExpand
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Exploring the Protein Stability Landscape: Bacillus subtilis Lipase A as a Model for Detergent Tolerance
A systematic study was conducted with Bacillus subtilis lipase A (BSLA) to determine the effect of every single amino acid substitution on detergent tolerance. BSLA is a minimal α/β‐hydrolase of 181Expand
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