Involvement of the Post-Transcriptional Regulator Hfq in Yersinia pestis Virulence
It appears that Hfq acts by controlling the expression of many virulence- and stress-associated genes, probably in conjunction with small noncoding RNAs, as a key regulator involved in Y. pestis stress resistance, intracellular survival and pathogenesis.
The Cyclic AMP Receptor Protein, CRP, Is Required for Both Virulence and Expression of the Minimal CRP Regulon in Yersinia pestis Biovar microtus
The notion that in addition to the reduced in vivo growth phenotype, the defect of pla expression in the crp mutant will greatly contribute to the huge loss of virulence of this mutant strain in subcutaneous infection is supported.
Cold-induced gene expression profiles of Vibrio parahaemolyticus: a time-course analysis.
A whole-genome DNA microarray was constructed to dissect expression profiles of Vibrio parahaemolyticus in response to a sudden temperature downshift and identified a 171-bp 5'-untranslated region in the cspA transcript.
Aspergillus as a versatile cell factory for organic acid production
A comparison of Agrobacterium-mediated transformation and protoplast-mediated transformation with CRISPR-Cas9 and bipartite gene targeting substrates, as effective gene targeting tools for…
The low-salt stimulon in Vibrio parahaemolyticus.
Effects of heterologous expression of phosphoenolpyruvate carboxykinase and phosphoenolpyruvate carboxylase on organic acid production in Aspergillus carbonarius
The production of citric acid increased in all the transformants in both glucose- and xylose-based media at pH higher than 3 but did not increase in the pH non-buffered cultivation compared with the wild type.
Enhanced succinic acid production in Aspergillus saccharolyticus by heterologous expression of fumarate reductase from Trypanosoma brucei
- Lei Yang, M. Lübeck, B. Ahring, P. S. Lübeck
- BiologyApplied Microbiology and Biotechnology
- 1 February 2016
This study demonstrates the feasibility of increasing succinic acid production through the cytosolic reductive pathway by genetic engineering in A. saccharolyticus.
Involvement of multiple influx and efflux transporters in the accumulation of cationic fluorescent dyes by Escherichia coli
High-throughput flow cytometry is used to assess the ability of individual gene knockout strains of E coli to take up two membrane-permeable, cationic fluorescent dyes, namely the carbocyanine diS-C3(5) and the DNA dye SYBR Green, concluding that the uptake of these dyes may be catalysed by a great many transporters of putatively broad and presently unknown specificity.
Prevention of influenza by targeting host receptors using engineered proteins
- H. Connaris, E. Govorkova, G. Taylor
- Biology, MedicineProceedings of the National Academy of Sciences
- 14 April 2014
It is shown that multivalent biologics, engineered using carbohydrate-binding modules specific for sialic acid, mask the cell-surface receptor recognized by the influenza virus and protect mice from a lethal challenge with 2009 pandemic H1N1 influenza virus.