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Geneious! Simplified genome skimming methods for phylogenetic systematic studies: A case study in Oreocarya (Boraginaceae)1
Genome skimming represents a much-improved primary data collection over PCR+Sanger sequencing when chloroplast DNA (cpDNA), nuclear ribosomal DNA (nrDNA), and mitochondrial DNA (mtDNA) are the target sequences.
Phylogeny of the popcorn flowers: Use of genome skimming to evaluate monophyly and interrelationships in subtribe Amsinckiinae (Boraginaceae)
Although genome skimming verifies the monophyly of many genera and clades of Amsinckiinae, relationships among those clades and along the backbone of the trees remain uncertain, their elucidation possibly a factor of short branch lengths and likely requiring different types of molecular data.
eDNA metabarcoding bioassessment of endangered fairy shrimp (Branchinecta spp.)
Results from ten Southern California vernal pools comparing eDNA and traditional dip-net methods showed that eDNA metabarcoding with 16S rDNA provides exceptional species-level resolution, and provide resource managers a simple, cost-effective, and non-invasive method for biomonitoring endangered fairy shrimp species.
From Pectinate to Divaricate: The Promise of NGS Genome Skimming for Phylogenetic Systematics of Oreocarya (Boraginaceae)
Although genome skimming provides a much-improved primary data collection method for phylogenetic resolution at the species level, and is suitable for systematic revision of Oreocarya at the sectional level, targeting of LCNG holds the most promise for a well-resolved phylogeny ofOreocarya in the future.