Lee A. Polikoff

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Cystic fibrosis (CF) is a lethal genetic disorder most commonly caused by the F508del mutation in the cystic fibrosis transmembrane conductance regulator (CFTR) gene. It is not readily amenable to gene therapy because of its systemic nature and challenges including in vivo gene delivery and transient gene expression. Here we use triplex-forming peptide(More)
Earlier work from this laboratory demonstrated a bumetanide-inhibitable K(+) uptake activity in cultured bovine lens epithelial cells, but not at the anterior surfaces of intact bovine lenses isolated in an Ussing-type chamber. Presently the distribution of the bumetanide-sensitive Na(+)-K(+)-2Cl(-) cotransporter within the lens was re-examined. To(More)
PURPOSE To characterize the effects of cAMP-elevating stimuli on the rabbit translens electrical parameters and examine the distribution of beta adrenoceptors about the epithelial surface. METHODS The electrophysiological experiments encompassed the isolation of lenses within a vertically arranged, Ussing-type chamber under short-circuit conditions, an(More)
Experimental maneuvers known to increase cellular cAMP levels evoked a stimulation in the K(+) influx across the anterior surfaces of isolated rabbit lenses, as measured by 86Rb(+) uptake. For this, the lenses were mounted in a modified Ussing-type chamber and exposed to the radiolabel under short-circuit conditions. The enhanced, cAMP-elicited flux was(More)
PURPOSE To determine if laser iridotomy altered the anterior segment anatomy of patients with plateau iris configuration. METHODS Twenty eyes of 9 female and 1 male patients were imaged using an ultrasound biomicroscope within 19 weeks before and 52 weeks after laser iridotomy. Measurements obtained included the anterior chamber depth (ACD),(More)
The relative distribution of acetylcholine (ACh) receptors on the surface of the isolated ocular lens of the rabbit was determined from induced changes in translens short-circuit current (I(SC)) and the translenticular resistance (R(t)) at seven delineated, parallel zones from the anterior to the posterior pole. For this, one O-ring (from among several(More)
PURPOSE To determine whether intraocular pressure (IOP) in the early postoperative period after trabeculectomy or combined phacoemulsification-trabeculectomy, augmented with antimetabolite, correlates with IOP at one year in surgeries considered to be successful at that time point. DESIGN Retrospective case series. METHODS A chart review of(More)
BACKGROUND The Pediatric Early Warning System (PEWS) was created to identify unstable patients before their deterioration. Rapid response teams (RRTs) were developed to assist with management of such patients. In 2009, our institution mandated the activation of RRTs if a PEWS score was elevated (ie, ≥5). OBJECTIVES The goal of this study was to examine(More)
PURPOSE To determine if the intraocular pressure (IOP) effect of pilocarpine at various concentrations is additive to that of bimatoprost and to assess the tolerability of this combination. METHODS This was a randomized, prospective trial of patients with IOP > 21 mm Hg following appropriate medication washout. For all visits IOP was measured at 9:00 AM(More)
PURPOSE OF REVIEW To review the current literature on venous thromboembolism (VTE) in critically ill children. RECENT FINDINGS There is an increasing concern for VTE and its complications in critically ill children. Critically ill children are at increased risk of thromboembolism because of the treatment that they are receiving and their underlying(More)