Lea Valinsky

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One of the first steps in characterizing an ecosystem is to describe the organisms inhabiting it. For microbial studies, experimental limitations have hindered the ability to depict diverse communities. Here we describe oligonucleotide fingerprinting of rRNA genes (OFRG), a method that permits identification of arrayed rRNA genes (rDNA) through a series of(More)
Sequence-based typing (SBT) for Legionella pneumophila (Lp) has dramatically improved Legionnaires’ disease (LD) cluster investigation. Microbial whole genome sequencing (WGS) is a promising modality for investigation but sequence analysis methods are neither standardised, nor agreed. We sought to develop a WGS-based typing scheme for Lp using de novo(More)
MOTIVATION Oligonucleotide fingerprinting of ribosomal RNA genes (OFRG) is a procedure that sorts rRNA gene (rDNA) clones into taxonomic groups through a series of hybridization experiments. The hybridization signals are classified into three discrete values 0, 1 and N, where 0 and 1, respectively, specify negative and positive hybridization events and N(More)
19 § These authors contributed equally to this work 20 21 22 2 ABSTRACT 1 2 One of the first steps in characterizing an ecosystem is to describe the organisms 3 inhabiting it. For microbial studies, experimental limitations have hindered the ability to depict 4 these diverse communities. Here we describe oligonucleotide fingerprinting of ribosomal RNA 5(More)
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