Lea-Franziska Kraus

  • Citations Per Year
Learn More
We studied the intracellular total, oxidized and reduced glutathione levels in thymus and spleen rat lymphocytes cultured with or without Con A and 2-mercaptoethanol (2-ME). After 48 h culture, the total glutathione level decreased and the oxidized glutathione level increased in the two types of unstimulated and stimulated cells. In the presence of 2-ME,(More)
The in vivo mitogenic responses to lipopolysaccharide or concanavalin A by spleen cells of mice exposed to 20 ppm nitrogen dioxide (NO2) for 96 hr, were evaluated. [3H]Thymidine incorporation after addition of either mitogen, was significantly lower in spleen cells from acutely NO2-exposed mice (NO2 SC) than from control mice, although cell viability was(More)
Glutathione is known to be an important parameter for ConA proliferative response of murine splenocytes. We studied the glutathione status of ConA-stimulated rat splenocytes during the early and late phase of the mitogenic response under low (FO2 7%) and standard (FO2 21%) oxygen concentrations. We determined the intracellular total, oxidized and reduced(More)
The effects of different normobaric oxygen concentrations (40, 60 and 95%) on the survival and the proliferative response to Con A of rat lymphoid cells were studied. Spleen, thymus and peripheral blood mononuclear cells were tested. We found that oxygen concentrations modulated the proliferative response independently of cell survival. The addition of(More)
Immunological cell functions were evaluated during 24, 48 and 96 h O2 exposure in C57Bl/6 mice. A normobaric O2 exposure resulted in depression of delayed type hypersensitivity (DTH) to oxazolone and Staphylococcus aureus antigens. This effect was proportional to the duration of O2 exposure. The antibody response of splenic cells was more rapidly (24 h O2(More)
Glutathione plays an important role in the lymphocyte mitogenic response. We have demonstrated that 2-ME increases the ConA proliferative response of rat splenocytes and in parallel, causes an enhancement of glutathione synthesis in these cells. On the other hand, 2-ME had the same action on the glutathione level of thymocytes during the late phase of their(More)
Time dependent response for hyperoxic exposure was determined in vitro on ConA proliferative response of rat splenocytes, peripheral blood mononuclear cells and thymocytes. The proliferative responses were evaluated after different lengths of hyperoxic exposure (12-72 h, FiO2 = 0.95). After 24 h oxygen exposure, the spleen cell viability assessed by dye(More)
Rat spleen cells were cultivated in 1 ATA pure oxygen. The mitogenic responses with Con A were evaluated at different time of exposure (12 to 72 hrs). There was a stimulation of the cells after 12 hrs of exposure, which diminished at 18 hrs, in spite of the fact that the viability of the cells remained unchanged till 24 hrs of exposure. The kinetics of the(More)