Layla J Barkal

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Matrix metalloproteinases (MMPs) and A Disintegrin and Metalloproteinases (ADAMs) are two related protease families that play key roles in matrix remodeling and growth factor ligand shedding. Directly ascertaining the proteolytic activities of particular MMPs and ADAMs in physiological environments in a non-invasive, real-time, multiplex manner remains a(More)
The microbial secondary metabolome encompasses great synthetic diversity, empowering microbes to tune their chemical responses to changing microenvironments. Traditional metabolomics methods are ill-equipped to probe a wide variety of environments or environmental dynamics. Here we introduce a class of microscale culture platforms to analyse chemical(More)
1 Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, 2 Carbone Cancer Center, University of Wisconsin-Madison, Madison, Wisconsin, United States of America, 3 Department of Chemistry, University of Washington, Seattle, Washington, United States of America, 4 Department of Urology, University(More)
Germination of spores into actively growing cells is a process essential for survival and pathogenesis of many microbes. Molecular mechanisms governing germination, however, are poorly understood in part because few tools exist for evaluating and interrogating the process. Here, we introduce an assay that leverages developments in microfluidic technology(More)
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