Lawrence J. Osborne

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Our entering freshmen have little experience with the underlying concepts of computer science and information technology, and they are not familiar with the process of absorbing new information through listening and reading and cooperating with other well-trained individuals to increase their knowledge and skills in a subject. Simply making group(More)
Various combinations of cytokines have profoundly different effects on inhibition of apoptosis and stimulation of self-renewal division of hematopoietic stem cells (HSC) in short-term, ex vivo culture. Our goal was to quantitate expansion of cells with a primitive CD34+ Thy-1+ phenotype, as well as cell cycling, division history, differentiation, and(More)
The effect of Z protein, bovine serum albumin and spermine on 1,2-diacylglycerol acyltransferase (DGAT) was investigated. DGAT was measured in the presence of [14C]-palmitoyl-CoA and 1,2-diacylglycerol dispersed in Tween 20. As noted earlier, the activation of DGAT was observed in the presence of either spermine or Mg2+, with spermine being more effective.(More)
Adipose cytosol treated with spermine showed an aggregation of a cytosolic component which was isolated by centrifugation at 16,000 X g for 20 min. The resultant pellet contained 10% of protein, 40% of lipid and over 75-97% of Mg2+-dependent phosphatidate phosphohydrolase and CTP:phosphocholine cytidylyltransferase activities present in the original(More)
A simple one-step filtration method is described to separate larger adipocytes from the smaller ones by using nylon screen (52 microM pore size). Adipocytes retained on the screen were larger (60-90 micrometers) compared with those that passed through the screen. By using this separation technique, activities of various enzymes involved in triacylglycerol(More)
Thioredoxin expression within the reproductive tissues of the female mouse was analysed during the oestrous cycle stages of dioestrus, oestrus and metoestrus by Western blot analyses and immunocytochemistry. From Western blot analyses the expression of thioredoxin was found to be increased in oestrus compared to dioestrus and metoestrus. Localisation of(More)
The properties of Mg2+-dependent and Mg2+-independent phosphatidate phosphohydrolase activities were investigated in different subcellular fractions in rat adipose tissue. Phosphatidate phosphohydrolase activity was measured in the presence of aqueous dispersed phosphatidate as substrate, and the release of inorganic phosphate was taken as a measure of(More)
In the present studies, we have made several unique observations. First, we have shown that cytosolic phosphatidate phosphohydrolase from adipose tissue subjected to butyl-agarose chromatography was resolved into four different components. These components, designated as passthrough (PT), D150, D250 and E, were present in the proportions of 51:7:24:16,(More)