Lawrence C. H. Wang

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1. Liver mitochondrial functions were evaluated in Richardson's ground squirrels during different phases of the annual hibernation cycle. 2. Arrhenius plots of succinate oxidase-linked H+ ejection, Ca2+ uptake and O2 consumption exhibited discontinuities (T c) near 21 °C in summer-active (July) ground squirrels and at 11–13 °C in warm (20 °C)- or cold (4(More)
Mammalian hibernators experience dramatic reductions in body temperature, metabolic rate, respiratory rate, and heart rate during hibernation. These changes are precisely controlled and reversible with only internally driven mechanisms, suggesting specific biochemical regulation. We present a model that integrates our observations of differential liver gene(More)
Six healthy adult males were fed four different diets to determine the effects of the quantity of fat (30% or 40% of energy as fat) and type of fat (polyunsaturated or saturated) on utilization of fatty acids. Each diet was fed for 15 d. The ratio of dietary polyunsaturated to saturated fat (P:S) was formulated at either 0.2 or 1.0 at both fat intakes.(More)
The Ca2+ uptake by isolated cardiac sarcoplasmic reticulum (SR) was compared between Richardson's ground squirrels and rats at 37, 25, 15, and 5 degrees C. The rate of SR Ca2+ uptake in ground squirrels was significantly higher than in rats over the temperature range. This marked species difference was observed over a Ca2+ concentration range from 0.1 to 10(More)
The effect of low temperature on the cytosolic free Ca2+ [( Ca2+]i) has been investigated in isolated ventricular myocytes from adult rats using the fluorescent probe Indo-1. The distribution of Indo-1 between the mitochondrial and cytoplasmic compartments was first determined in the isolated myocytes using the digitonin and Triton X-100 treatments. By(More)
The thermogenic activity [mitochondrial guanosine 5'-diphosphate (GDP) binding] and capacity (uncoupling protein concentration, cytochrome oxidase activity) of brown adipose tissue have been investigated at different phases of the seasonally linked hibernation cycle in Richardson's ground squirrel. The amount of axillary brown adipose tissue and the total(More)
The rate of calcium uptake and the level of calcium accumulation was measured in cardiac muscle SR from hibernating and nonhibernating Richardson's ground squirrels. In whole heart homogenates, the rate of calcium uptake was higher (P less than 0.05) in hibernating animals than it was in active animals. Further purification of homogenates into sacroplasmic(More)
alpha 2-Macroglobulin, a major acute phase reactant in many species, increases in the Richardson's ground squirrel (Spermophilus richarsonii) during hibernation at the level of both mRNA and protein. To determine if the pattern of liver gene expression known as the acute phase response is elicited as a normal part of the physiological adaptation of(More)
The effects of cold hypoxia were examined during a time-course at 2 °C on levels of glycolytic metabolites: glycogen, glucose, glucose-1-phosphate, glucose-6-phosphate, fructose-6-phosphate, fructose-1,6-bisphosphate, phosphoenolpyruvate, pyruvate, lactate and energetics (ATP, ADP, AMP) of livers from rats and columbian ground squirrels. Responses of(More)
A novel isoform of calsequestrin was identified in sarcoplasmic reticulum vesicles from myocardial tissue of two species of hibernating ground squirrel. The protein was identified as calsequestrin by its cross-reactivity with antibodies raised against bovine cardiac calsequestrin, its pH-sensitive mobility in sodium dodecylsulphate-polyacrylamide gels,(More)