Lavanya Pothana

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Death of immature animals is one of the reasons for the loss of genetic diversity of rare and endangered species. Because sperm cannot be collected from immature males, cryobanking of testicular tissue combined with testis xenografting is a potential option for conservation. The objective of this study was to evaluate the establishment of spermatogenesis in(More)
Buffalo calves have a high mortality rate (~80%) in commercial dairies and testis cryopreservation can provide a feasible option for the preservation of germplasm from immature males that die before attaining sexual maturity. The aim of the present study was to evaluate combinations of 10 or 20% dimethylsulfoxide (DMSO) with 0, 20 or 80% fetal bovine serum(More)
For primates that may unexpectedly die, spermatozoa recovered from the epididymis can be used for assisted reproductive technologies (ART). However, in cases of unsuccessful epididymal sperm retrieval, adult testes are the only source of sperm. This study reports cryopreservation of testicular tissue of three adult primates (mandrill, chimpanzee, and(More)
Ectopic autografting of testis tissue is a promising approach for studying testicular development, male germline preservation and restoration of male fertility. In this study, we examined the fate of various testicular cells in adult mouse testes following ectopic autografting at 1, 2, 4 and 8 weeks post grafting. Histological examination showed no evidence(More)
Cryopreservation of immature testis is a feasible approach for germplasm preservation of male animals. Combinations of dimethyl sulfoxide (DMSO) and foetal bovine serum (FBS) are used for testis cryopreservation. However, an alternative to FBS is needed, because FBS is expensive. Buffalo ocular fluid (BuOF), a slaughter house by-product, could be an(More)
Ectopic xenografting of testis is a feasible option for preservation of male fertility and angiogenesis plays a pivotal role in xenograft survival and functionality. When compared to immature testis, the adult testis is unable to establish functional xenografts due to potentially lower efficiency to induce angiogenesis. The precise molecular mechanism,(More)
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