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OBJECTIVE To isolate and characterize mesenchymal stem cells (MSCs) from canine muscle and periosteum and compare proliferative capacities of bone marrow-, adipose tissue-, muscle-, and periosteum-derived MSCs (BMSCs, AMSCs, MMSCs, and PMSCs, respectively). SAMPLE -7 canine cadavers. PROCEDURES -MSCs were characterized on the basis of morphology,(More)
OBJECTIVE To characterize equine muscle tissue- and periosteal tissue-derived cells as mesenchymal stem cells (MSCs) and assess their proliferation capacity and osteogenic potential in comparison with bone marrow- and adipose tissue-derived MSCs. SAMPLE Tissues from 10 equine cadavers. PROCEDURES Cells were isolated from left semitendinosus muscle(More)
The in vitro viability, osteogenic differentiation, and mineralization of four different equine mesenchymal stem cells (MSCs) from bone marrow, periosteum, muscle, and adipose tissue are compared, when they are cultured with different collagen-based scaffolds or with fibrin glue. The results indicate that bone marrow cells are the best source of MSCs for(More)
Stem cell therapy and cell-based therapies using other progenitor cells are becoming the treatment of choice for many equine orthopedic lesions. Important criteria for obtaining autogenous equine progenitor cells in vitro for use in clinical cell-based therapy include the ability to isolate and expand cells repeatedly to high numbers (millions) required for(More)
Autologous mesenchymal stem cells (MSCs) have been used as a potential cell-based therapy in various animal and human diseases. Their differentiation capacity makes them useful as a novel strategy in the treatment of tissue injury in which the healing process is compromised or delayed. In horses, bone healing is slow, taking a minimum of 6–12 months. The(More)
The compressive, bending and torsional mechanical properties of osteotomized adult equine tibiae stabilized with an interlocking intramedullary nail (nail-tibia composite) were compared with those of intact tibiae to determine the clinical applicability of the the nail for repair of tibial fractures in adult horses. The mean yield load, failure load, and(More)
OBJECTIVE To compare the efficacy of osteoprogenitors in fibrin glue to fibrin glue alone in bone healing of surgically induced ostectomies of the fourth metacarpal bones in an equine model. STUDY DESIGN Experimental. ANIMALS Adult horses (n = 10). METHODS Segmental ostectomies of the 4th metacarpal bone (MC4) were performed bilaterally in 10 horses.(More)
Sacral fractures in 2 dairy cattle were repaired surgically for cosmetic reasons. A heifer had a Salter-Harris type-I fracture of the fifth sacral vertebra, which was repaired with a 4.5-mm narrow dynamic compression plate. A cow had complete fracture of the fourth sacral body, which was repaired with 2 extra-large plastic spinous process plates. Both(More)
OBJECTIVE To determine the monotonic mechanical properties of osteotomized adult equine tibiae stabilized with two dynamic compression plates (DCP) and to compare the mechanical properties with those of intact tibiae and in vivo loads. STUDY DESIGN The compressive, bending, and torsional mechanical properties of plated and intact tibiae were assessed in(More)
Bone cell cultures were evaluated to determine if osteogenic cell populations at different skeletal sites in the horse are heterogeneous. Osteogenic cells were isolated from cortical and cancellous bone in vitro by an explant culture method. Subcultured cells were induced to differentiate into bone-forming osteoblasts. The osteoblast phenotype was confirmed(More)