Laurence K. Grill

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A linear mitochondrial plasmid reported to be associated with cytoplasmic male sterility in the genus Brassica was analyzed. A protein was found to be associated with the 5′ ends of the plasmid. The entire plasmid was cloned by the homopolymer tailing technique via free hydroxyl groups present at its 3′ ends. DNA sequence analysis of the cloned plasmid(More)
Using malaria as a model disease, we engineered the surface of tobacco mosaic tobamovinis (TMV) for presentation of selected epitopes to the mammalian immune system. The TMV coat protein is a well-characterized and abundant self-assembling polymer previously shown to be a highly immunogenic carrier. Selected B-cell epitopes were either inserted into the(More)
A recombinant Saccharomyces cerevisiae, expressing and secreting rice α-amylase, converts starch to ethanol. The rice α-amylase gene (OS103) was placed under the transcriptional control of the promoter from a newly described Pichia pastoris alcohol oxidase genomic clone. The nucleotide sequences of ZZA1 and other methanol-regulated promoters were analyzed.(More)
The trait of cytoplasmic male sterility, expressed in plants bearing the 447 cytoplasm of Vicia faba, is uniquely and positively correlated with the presence of a linear double-stranded RNA molecule (dsRNA) 16.7 kb in size. Restriction enzyme digestion profiles of mitochondrial DNA isolated from fertile and cytoplasmic malesterile (CMS) lines do show a(More)
The current anthrax vaccine requires improvements for rapidly invoking longer-lasting neutralizing antibody responses with fewer doses from a well-defined formulation. Designing antigens that target neutralizing antibody epitopes of anthrax protective antigen, a component of anthrax toxin, may offer a solution for achieving a vaccine that can induce strong(More)
We have cloned and functionally expressed a tyrosinase gene from Streptomyces antibioticus in Escherichia coli under the control of an inducible bacteriophage T7 promoter. Recombinant E. coli cells containing the induced tyrosinase gene produced melanin pigments on agar plates and in liquid culture when supplemented with copper and tyrosine. The expression(More)
STEPHEN J. GARGER, THOMAS TURPEN (Dep't. of Molecular Biology, Zoecon Corporation, 975 California Avenue, Palo Alto, CA 94304), JAMES C. CARRINGTON, T. JACK MORRIS (Dep't. of Plant Pathology, University of Califorma, Berkeley, CA 94720), RAMON L. JORDAN, J. ALLAN DODDS (Dep't. of Plant Pathology, University of California, Riverside, CA 95121), and LAURENCE(More)
p lant viruses have a number of traits that may make them useful for the improvement of aericultural crops. The value of some plant viruses in horticultural applications has already been demonstrated, but some problems remain to be soh'ed before such agents can bc used in agricultural practice. A number of known useful viral induced symptoms will herein be(More)
c esium chloride density gradients have frequently been used for the purification of encapsidated viruses and nucleic acids. However, such gradients in fixed angle rotors have required the sole commItment of an ultracentrifuge for man}' hours (or days). We have found that by using higher rotor speeds and a simple two-step layering technique, we can achieve(More)
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