Lauren Sloan

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Calcitonin gene-related peptide (CGRP) is a neuropeptide that is released at the neuromuscular junction in response to nerve excitation. To examine the relationship between plasma CGRP concentration and intracellular glucose metabolism in conscious rats, we performed insulin (22 pmol.kg-1.min-1) clamp studies combined with the infusion of 0, 20, 50, 100,(More)
Background Targeting Dendritic Cells (DCs) with anti-DC receptor antibody-antigen fusion proteins represents a novel approach to vaccine development. In mouse models, these innovative vaccines induce enhanced cellular, humoral, or mixed immune responses. Targeting antigens to particular DC subsets can lead to distinct immune outcomes but the consequence of(More)
Background Identification of promiscuous antigenic regions is crucial for the design of an epitope-based vaccine. Still, the quality of the responses has been under estimated in most cases when only IFN-γ is used to measure anti-HIV cellular immunity. Luminex multiplexing system allows the identification of T cell responses characteristic of T cell subtypes(More)
Opioid receptors exist in at least three forms: mu, delta and kappa. Agonists at mu receptors produce orthostatic hypotension in man by a mechanism involving a reduction in baroreflex sensitivity. We describe here the cardiovascular properties of metkephamid, a relatively selective delta opioid receptor agonist. Blood pressure, heart rate and plasma(More)
Sodium bisulfite reacted with unsaturated fatty acids, significantly increasing their polarity as determined by behavior on silica gel thin-layer chromatography. The ultraviolet absorption spectra of unsaturated fatty acids (due to the presence of double bonds) were abolished as a result of the reaction. Fatty acids containing more than one double bond(More)
Background Antibodies directed against antigen-presenting cell (APC) receptors can deliver antigens very efficiently. This receptor-targeting vaccine system facilitates the uptake, processing and presentation of extracellular antigens. In our approach, an antibody against human CD40 was fused to HIV antigens and these prototype vaccines were tested for(More)
Background Previously described highly immunogenic epitopes from Gag are able to stimulate Th1/CTL cells. This response is essential for controlling progression of HIV infection. Techniques like IFN-γ-ELISPOT and IFN-γ intracellular staining are currently the standard methods to define antigen-specific immune responses. A recent method, the Luminex analysis(More)
Methods 19 pts with CD4 >500 cells/m3 and HIV RNA <50 cp/ml under HAART received at W0, 4, 8 and 12 ex-vivo generated IFN-a DC loaded with HIV-1 lipopeptides. Analytical treatment interruption (ATI) was conducted from W24. HAART resumption regardless of the reason and CD4 <350 cells/mm3 (or <25%) were considered as end points. HIV-specific immunity was(More)
Background Clinical trials have shown that dendritic cell (DC)-based vaccines can induce antigen-specific immune responses as well as clinical responses in patients with stage IV cancer. In this study, a monocyte-derived DC vaccine pulsed with HIV antigen lipopeptides (LIPO5) was developed in preparation for a pilot vaccine clinical trial (DALIA) aimed at(More)