Lauren M Junker

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Escherichia coli strain PHL628 was subjected to saturating Tn5 transposon mutagenesis and then grown under competitive planktonic or biofilm conditions. The locations of transposon insertions from the remaining cells were then mapped on a gene array. The results from the array mapping indicated that 4.5 % of the E. coli genome was important under these(More)
Phages have recently been implicated as important in biofilm development, although the mechanisms whereby phages impact biofilms remain unclear. One defective lambdoid phage carried by Escherichia coli K-12 is DLP12. Among the genes found in DLP12 are essD, ybcS and rzpD/rzoD, which are homologues of the Lambda phage genes encoding cell-lysis proteins (S, R(More)
The biofilm-specific gene expression of Escherichia coli PHL628 was compared with that from exponentially growing planktonic cells using macroarray technology. In duplicate experiments, both biofilm and planktonic cells were grown in separate continually stirred tank reactors at 57% of the maximal planktonic growth rate. When transcriptional results from(More)
OBJECTIVE This study compared the attached biofilm populations on acrylonitrile-butadiene-styrene (ABS) plastic with and without the incorporation of the antimicrobial triclosan [5-chloro-2-(2,4-dichlorophenoxy) phenol] after 1-3 weeks of exposure to drinking water. METHODS Biofilms were cultivated on triclosan-incorporated (TP) and control plastics (CP)(More)
Pseudomonas putida F1 cannot grow on styrene despite being able to degrade it through the toluene degradation (tod) pathway. Previous work had suggested that this was because TodF, the meta-fission product (MFP) hydrolase, was unable to metabolize the styrene MFP 2-hydroxy-6-vinylhexa-2,4-dienoate. Here we demonstrate via kinetic and growth analyses that(More)
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