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Crystal structure of the chemokine receptor CXCR4 in complex with a viral chemokine
TLDR
The structure helped rationalize a large body of mutagenesis data and together with modeling provided insights into CXCR4 interactions with its endogenous ligand CXCL12, its ability to recognize diverse ligands, and the specificity of CC and CXC receptors for their respective chemokines. Expand
Crystal structure of the anti-viral APOBEC3G catalytic domain and functional implications
TLDR
The high-resolution crystal structure of the carboxy-terminal deaminase domain of APOBEC3G (APOBec3G-CD2) purified from Escherichia coli is reported, and residues involved in substrate specificity, single-stranded DNA binding and deaminases activity are identified. Expand
WRN exonuclease structure and molecular mechanism imply an editing role in DNA end processing
TLDR
Results indicate WRN-exo is a human DnaQ family member and support DnaZ-like proofreading activities stimulated by Ku70/80, with implications for WRN functions in age-related pathologies and maintenance of genomic integrity. Expand
The structure of a DnaB-family replicative helicase and its interactions with primase
TLDR
The full-length crystal structure of G40P, a DnaB family helicase, reveals an unusual architectural feature and a new type of assembly mechanism within the hexamer complex, and structure-guided mutational studies indicate an important role for the N-terminal tier in binding primase and regulating primase-mediated stimulation of helicase activity. Expand
Stoichiometry and geometry of the CXC chemokine receptor 4 complex with CXC ligand 12: Molecular modeling and experimental validation
TLDR
The observation of a 1:1 stoichiometry is in line with accumulating evidence supporting monomers as minimal functional units of G protein-coupled receptors, and suggests transmission of conformational changes across the dimer interface as the most probable mechanism of altered signaling by receptor heterodimers. Expand
Associating protein activities with their genes: rapid identification of a gene encoding a methylglyoxal reductase in the yeast Saccharomyces cerevisiae
TLDR
It is shown that methylglyoxal reductase (NADPH‐dependent) is encoded by GRE2 (YOL151w) and associated this activity with its gene by partially purifying the enzyme and identifying by MALDI–TOF the proteins in candidate bands on SDS–PAGE gels whose relative intensities correlated with specific activity through three purification steps. Expand
Disulfide Trapping for Modeling and Structure Determination of Receptor: Chemokine Complexes.
TLDR
Method for generating irreversible covalent binary protein complexes from unbound protein partners by introducing two cysteine residues, one per interaction partner, at selected positions within their interaction interface is described. Expand
Improving the Expression and Purification of Soluble, Recombinant Native-Like HIV-1 Envelope Glycoprotein Trimers by Targeted Sequence Changes
TLDR
This work shows how to produce trimers from a clade C virus, CZA97.664, by using an array of protein engineering techniques to improve a prototypic construct and shows that the methods may have wider utility for other env genes, thereby further guiding immunogen design. Expand
Crosslinking-guided geometry of a complete CXC receptor-chemokine complex and the basis of chemokine subfamily selectivity
TLDR
This work establishes the existence and the structure of a novel interface between the CXCR4 distal N-terminus and CXCL12 β1-strand and suggests the possibility of new affinity and signaling determinants, and fills a critical void on the structural map of an important class of therapeutic targets. Expand
Characterization of a stable HIV-1 B/C recombinant, soluble, and trimeric envelope glycoprotein (Env) highly resistant to CD4-induced conformational changes
TLDR
An HIV-1 B/C recombinant, native-like trimeric Env protein that is highly resistant to CD4-induced conformational changes but displays epitopes recognized by a diverse array of bnAbs is reported on. Expand
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