Laura Maria Costantina Forino

  • Citations Per Year
Learn More
The pattern of DNA and RNA puffs in pair VII of polytene chromosomes has been investigated in the suspensor ofPhaseolus coccineus during early embryo development. The pattern of3H-TdR and3H-U incorporation has been also detected. Collected data indicate that: 1. both heterochromatic regions, p11 and q(111+112), of chromosome pair VII, organize large DNA(More)
In the giant cells of thePhaseolus coccineus embryo suspensor the number of nucleoli is not constant during early embryogenesis. Over the same period the number and the type of chromosomes involved in the nucleolar organization also vary. Moreover, the percentage of DNA and RNA puffs organized by ribosomal cistron (rc) bearing regions changes. When single(More)
BACKGROUND AND AIMS Plant adaptation to submergence can include the formation of prominent aerenchyma to facilitate gas exchange. The aim of this study was to characterize the differentiation of the constitutive aerenchyma in the stem of the aquatic macrophyte Egeria densa (Hydrocharitaceae) and to verify if any form of cell death might be involved. (More)
DNA was extracted from etiolated plantlets ofPhaseolus coccineus L. and studied by means of thermal denaturation and reassociation kinetics and CsCl analytical ultracentrifugation. A DNA fraction reassociating within an equivalent Cot of 1.15 × 10−1 (fast renaturing DNA sequences; FRS) was found to constitute 20.8% of the total DNA, 6.8% of which is(More)
Cadmium uptake, tissue localization and structural changes induced at cellular level are essential to understand Cd tolerance in plants. In this study we have exposed plants of Pteris vittata to different concentrations of CdCl2 (0, 30, 60, 100 μM) to evaluate the tolerance of the fern to cadmium. Cadmium content determination and its histochemical(More)
On the assumption that arsenic induces stress morphogenetic responses involved in As tolerance and hyperaccumulation in the Pteris vittata fern, we analyzed the root system of young sporophytes grown in 250, 334, and 500 μM As for five days and for 14 days. Anatomical and histological analyses were performed in plants grown for five days to evaluate the(More)
When exposed to an acute ozone stress, cell death occurred in leaves of the O3 sensitive Populus deltoides × maximowiczii clone Eridano. After treatment (5 h fumigation and 24 h recovery), the damaged areas covered more than 50 % of the leaf surface. At cellular level, these lesions were preceded by some apoptotic hallmarks and by biochemical and(More)
Pollen grains of dehiscent anthers of two cultivars ofMalus domestica “Golden Delicious” (partially self-incompatible) and “Starkrimson” (self-incompatible) were studied by Feulgen cytophotometry and starch staining. The collected results show: I) in both cultivars generative cells in G1 (DNA content/nucleus 1 c) and in G2 (DNA content/nucleus 2 c) are(More)
RNA synthesis in giant cells containing polytene chromosomes in the embryo suspensor ofPhaseolus coccineus was analyzed by autoradiography after [3H]-uridine treatment. Embryos at the heart-shaped stage of development and at a cotyledonary stage were studied. Discontinuous labelling of the polytene chromosomes was always observed. The chromosomes were(More)
Different regions of polytene chromosomes pair VI have been characterized by: 1. morphological observations, 2. incorporation of3H-thymidine and3H-uridine, 3. cytophotometry of DNA and associated proteins, 4. hybridization with satellite DNA and highly repeated DNA sequences. The collected data indicate that DNA and RNA puffs are organized by(More)