Laura Giagnoni

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It is still problematic to use enzyme activities as indicators of soil functions because: (1) enzyme assays determine potential and not real enzyme activities; (2) the meaning of measured enzyme activities is not known; (3) the assumption that a single enzyme activity is an indicator of nutrient dynamics in soil neglects that the many enzyme activities are(More)
We studied the molecular diversity of β-glucosidase-encoding genes, microbial biomass, cellulase, N-acetyl-glucosaminidase, β-glucosidase, and β-galactosidase activities in the rhizosphere and bulk soil of two maize lines differing in nitrogen use efficiency (NUE). The maize lines had significant differences in diversity of β-glucosidase-encoding genes in(More)
We studied the responses of soil microbial biomass, respiration and enzyme activities to temperature in three Mexican soils. Soils were incubated at temperature range of 15–550 °C at 50 (wet) and 10 % (dry) of their water holding capacity. Soils were assayed for their adenosine triphosphate (ATP) content, CO2-C evolution and acid and alkaline(More)
Study of the changes in soil microbial biomass, enzyme activity and the microbial community structure in the rhizosphere of two contrasting maize lines differing in the nitrogen use efficiency (NUE). The Lo5 and T250 inbred maize characterized by high and low NUE, respectively, were grown in rhizoboxes allowing precise sampling of rhizosphere and bulk soil(More)
Soil proteomics is facing problems such as low yields of protein extraction from soil and low protein identification rates as compared to theoretical estimates of soil proteome. This work aimed to evaluate the effect of soil-borne humic substances (HS) on the identification of model proteins with different properties, such as myoglobin (Mb), α-glucosidase(More)
Whole-cell biosensors are natural or engineered microorganisms producing signals in response to specific stimuli. This review introduces the use of whole-cell biosensors for the study of the soil system, discuss the recent developments and some current limitations and draws future prospects of the whole-cell biosensors for application to the study of the(More)
We studied the microbial functional diversity, biochemical activity, heavy metals (HM) availability and soil toxicity of Cd, Pb and Zn contaminated soils, kept under grassland or short rotation coppice (SRC) to attenuate the risks associated with HM contamination and restore the soil ecological functions. Soil microbial functional diversity was analyzed by(More)
The rate of nitrate (NO3 −) uptake and changes in rhizosphere properties were studied growing seedlings of two maize inbred lines differing in nitrogen use efficiency (NUE) in rhizoboxes. Changes in NO3 − uptake rates occurred in response to anion addition (induction) in seedlings grown both in hydroponic culture and in soil in rhizoboxes. The(More)
We evaluated the potential of a phytoremediated sediment (TR) dredged from maritime port as peat-free growth substrate for seven ornamental plants, in comparison with an untreated sediment (NT), in a greenhouse experiment. The studied plants were Quercus ilex, Photinia x fraseri, Viburnum tinus, Cistus albidus, Raphiolepis indica, Westringia fruticosa and(More)
We studied the effects of humic substances (HS) extracted from soil on the identification of the recombinant ovine prion protein (RecPrP) by denaturing (sodium dodecyl sulfate polyacrylamide gel electrophoresis [SDS-PAGE]) and native PAGE (N-PAGE), and mass spectrometry (MS), at various HS to RecPrP contact ratios. The results showed that the contact with(More)