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Use of the green fluorescent protein (Gfp) from the jellyfish Aequorea victoria is a powerful method for nondestructive in situ monitoring, since expression of green fluorescence does not require any substrate addition. To expand the use of Gfp as a reporter protein, new variants have been constructed by the addition of short peptide sequences to the(More)
As a representative member of the toluene-degrading population in a biofilter for waste gas treatment, Pseudomonas putida was investigated with a 16S rRNA targeting probe. The three-dimensional distribution of P. putida was visualized in the biofilm matrix by scanning confocal laser microscopy, demonstrating that P. putida was present throughout the(More)
A fast routine method for estimating bacterial cell growth rates by using the metachromatic dye acridine orange is described. The method allows simultaneous estimates of cellular RNA and DNA contents of single cells. Acridine orange staining can be used as a nonspecific supplement to quantitative species-specific hybridizations with fluorescence-labelled(More)
An avirulent, streptomycin-resistant Salmonella typhimurium strain, SL5319, and its lipopolysaccharide (LPS)-deficient mutant strain, SL5325, differ in their ability to colonize the large intestines of streptomycin-treated mice. When fed to mice independently, the strains colonize equally well, but when fed together, the LPS-deficient mutant is outcompeted(More)
A computerized method for automatic evaluation and comparison of crossed immunoelectrophoretic and crossed radioimmunoelectrophoretic patterns that requires limited hardware resources has been developed. For the initial reading of the plates an ordinary video camera is used. Feature extractors that allow the computer to recognize a point on the(More)
This paper reports on the process and outcomes of big data analytics of ride records for Green cabs and Uber in the outer boroughs of New York City (NYC), USA. Uber is a new entrant to the taxi market in NYC and is rapidly eating away market share from the NYC Taxi & Limousine Commission's (NYCTLC) Yellow and Green cabs. The problem investigated(More)
The possibilities for implementation of a sliding mode algorithm for training multilayer NN proposed in [12] as an online mechanism for adaptation in closed-loop feedback neurocontrol systems have been investigated. It is confirmed that the algorithm can be used to train the network structures as they interact with the external environment. The learning(More)
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