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Cell cycle progression was studied in serum-free batch cultures of Spodoptera frugiperda (Sf9) insect cells, and the implications for proliferation and productivity were investigated. Cell cycle dynamics in KBM10 serum-free medium was characterized by an accumulation of 50-70% of the cells in the G(2)/M phase of the cell cycle during the first 24 h after(More)
1H/15N and 13C NMR were used to investigate metabolism in Spodoptera frugiperda (Sf9) cells. Labelled substrates ([2-15N]glutamine, [5-15N]glutamine, [2-15N]glutamate, 15NH4Cl, [2-15N]alanine, and [1-13C]glucose) were added to batch cultures and the concentration of labelled excreted metabolites (alanine, NH4+, glutamine, glycerol, and lactate) were(More)
Here we describe the three-dimensional crystal structures of human glucocorticoid receptor ligand-binding domain (GR-LBD) in complex with the antagonist RU-486 at 2.3 A resolution and with the agonist dexamethasone ligand together with a coactivator peptide at 2.8 A. The RU-486 structure was solved in several different crystal forms, two with helix 12(More)
The physiology of cultured animal cells, in particular hybridoma, myeloma and insect cells, with respect to growth and proliferation, amino acid metabolism, energy metabolism and cellular responses to environmental stress is discussed in this paper. The rate of proliferation of hybridoma cells in serum-containing media is limited by growth factors at a(More)
Experiments were carried out to begin to define the behavioral functions of the lateral suprasylvian (LS) visual area of the cat's cortex. Behavioral tasks were chosen for analysis on the basis of previous suggestions in the literature concerning possible functions of LS cortex and its afferent pathways. These tasks included the ability of cats to orient(More)
Substrate-limited fed-batch cultures were used to study growth and overflow metabolism in hybridoma and insect cells. In hybridoma cells a glucose-limited fed-batch culture decreased lactate formation but increased glutamine consumption and ammonium formation. Glutamine limitation decreased ammonium and alanine formation but did not enhance glucose(More)
Twenty eyes in 20 patients with herpetic keratitis were treated with excimer laser photoablation. Sixteen eyes underwent phototherapeutic keratectomy because of scars and surface irregularities, with the goal to improve vision and 1 eye for cosmetic reasons. Two eyes had slight scars but were treated because of a refractive error (PRK). One eye had a(More)
The tartrate-resistant acid phosphatase (TRAP) of rat osteoclasts has been shown to exhibit high (85-94%) identity at the amino acid sequence level with the purple acid phosphatase (PAP) from bovine spleen and with pig uteroferrin. These iron-containing purple enzymes contain a binuclear iron centre, with a tyrosinate-to-Fe(III) charge-transfer transition(More)
Vocal abuse is the best-known etiological factor in contact ulcer. Other factors, such as hiatus hernia and gastroesophageal reflux have been discussed. A 12-year study of 58 male patients (mean age 52 years) with present or previous contact ulcers, is reported. Forty-three patients were investigated with esophageal function tests, ie, esophageal manometry(More)
One hundred sixty-six eyes with corneal disease were treated with the excimer laser in order to ablate opacities, irregularities, or to improve wound healing. Postoperative follow up time was 15 months (range 6 to 28 months). An individual goal was set for each patient and 84% achieved the goal. The visual acuity change is given for each subgroup.(More)