Lars Gerdes

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Digital PCR in droplets (ddPCR) is an emerging method for more and more applications in DNA (and RNA) analysis. Special requirements when establishing ddPCR for analysis of genetically modified organisms (GMO) in a laboratory include the choice between validated official qPCR methods and the optimization of these assays for a ddPCR format. Differentiation(More)
Real-time PCR used to be the gold standard when it comes to detection of rare mutations, copy number variations or genetically modified organisms. A new option for DNA analyses is the digital PCR. In digital PCR, the reaction mix is distributed to many partitions and endpoint PCR is performed. The fraction of positive partitions can be used to calculate the(More)
According to Regulation (EU) No 619/2011, trace amounts of non-authorised genetically modified organisms (GMO) in feed are tolerated within the EU if certain prerequisites are met. Tolerable traces must not exceed the so-called ‘minimum required performance limit’ (MRPL), which was defined according to the mentioned regulation to correspond to 0.1% mass(More)
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