Lars Gerdes

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Digital PCR in droplets (ddPCR) is an emerging method for more and more applications in DNA (and RNA) analysis. Special requirements when establishing ddPCR for analysis of genetically modified organisms (GMO) in a laboratory include the choice between validated official qPCR methods and the optimization of these assays for a ddPCR format. Differentiation(More)
Real-time PCR used to be the gold standard when it comes to detection of rare mutations, copy number variations or genetically modified organisms. A new option for DNA analyses is the digital PCR. In digital PCR, the reaction mix is distributed to many partitions and endpoint PCR is performed. The fraction of positive partitions can be used to calculate the(More)
Mit der klassischen Polymerase-Kettenreaktion (Polymerase Chain Reaction, PCR) werden bestimmte Zielabschnitte des Erbgutes (DNA) in einem einzigen Reaktionsansatz gezielt vervielfältigt und nachgewiesen. Bei der sogenannten droplet digital PCR (ddPCR) wird der Reaktionsansatz auf zehntausende winzige Tröpfchen (kleiner als 1 nL) verteilt(More)
Digital PCR (dPCR), as a new technology in the field of genetically modified (GM) organism (GMO) testing, enables determination of absolute target copy numbers. The purpose of our study was to test the transferability of methods designed for quantitative PCR (qPCR) to dPCR and to carry out an inter-laboratory comparison of the performance of two different(More)
According to Regulation (EU) No 619/2011, trace amounts of non-authorised genetically modified organisms (GMO) in feed are tolerated within the EU if certain prerequisites are met. Tolerable traces must not exceed the so-called ‘minimum required performance limit’ (MRPL), which was defined according to the mentioned regulation to correspond to 0.1% mass(More)
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