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Cytokinins (CKs) coming from the roots via the xylem are known to delay leaf senescence, and their decline may be important in the senescence of soybean (Glycine max) plants during pod development (monocarpic senescence). Therefore, using radioimmunoassay of highly purified CKs, we quantified the zeatin (Z), zeatin riboside (ZR), the dihydro derivatives(More)
When [(3)H]dihydrozeatin riboside and [(3)H]zeatin riboside were supplied to soybean (Glycine max L.) explants (comprising one leaf, associated pods, and subtending stem) via the xylem at mid to late podfill, 0.1% of the supplied (3)H was extracted from the seeds. The distribution of (3)H in the explants was similar to that bound previously following uptake(More)
Like most monocarpic plants, longevity of Arabidopsis thaliana plants is controlled by the reproductive structures; however, they appear to work differently from most dicots studied. Neither male- and female-sterility mutations (ms1-1 and bell1, respectively) nor surgical removal of the stems with inflorescences (bolts) at various stages significantly(More)
Chromatin has been prepared from nuclei isolated from the dinoflagellate alga Gyrodinium cohnii. This chromatin contains RNA, acid-insoluble proteins, and acid-soluble proteins; the respective ratios to amount of DNA are about 0.09, 0.48, 0.08 (by weight). Not only is the amount of acid-soluble protein associated with the DNA much less than it is in the(More)
Chromatin was prepared by two dif ferent methods f rom isolated nuclei of Gyrodinium cohnii ( Cryptothecodinium cohnii) and Peridinium trochoideum. These isolation procedures are different from those generally used to prepare eukaryote chromatin, because the latter do not work for dinoflagellate chromatin. The chemical composition of this chromatin is(More)
Soybean plants (Glycine max [L.] Merr. cv Clark) carrying nuclear and cytoplasmic "stay-green" mutations, which affect senescence, were examined. Normally, the levels of chlorophyll (Chl) a and b decline during seedfill and the Chl a/b ratio decreases during late pod development in cv Clark. Plants homozygous for both the d(1) and d(2) recessive alleles, at(More)
The intracellular binding of dinitroaniline herbicides was studied in order to analyze the mechanism of their colchicine-like action. When corn root apices (5 millimeters) are incubated in [(14)C]oryzalin (a dinitroaniline herbicide), the (14)C is taken up rapidly, reaching a plateau in about 4 hours, which corresponds to the minimum incubation time in(More)