Larry C. Stoner

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STONER, L. C., M. B. BURG, AND J. ORLOFF. Ion transport in cortical collecting tubule; effect of amiloride. Am. J. Physiol. 227(2) : 453-459. 1974.-T b 1 u u es were dissected from rabbit kidneys and perfused in vitro. In cortical collecting tubules the transepithelial electrical potential difference (PD) was 35 mV (lumen negative). There was net active(More)
The mechanism of Ca2+ transport by various segments of the distal nephron was studied in vitro using the isolated perfused tubule technique. Calcium absorption in the distal convoluted tubule (DCT) and the granular portion of the cortical collecting duct (CCTg) was significantly enhanced in the presence of parathyroid hormone (PTH), 3 X 10(-2) U/ml. Na+ was(More)
A technique is described whereby it is possible to evert a 0.5- to 1.0-mm segment of an amphibian renal tubule and perfuse it in vitro. Consequently, the apical membranes of an intact nephron fragment are directly accessible for electrophysiological study. Viability of the cells of everted diluting segments taken from Ambystoma kidney was indicated by 1)(More)
Studies on isolated perfused tubules of the tiger salamander (Ambystoma tigrinum) have shown that the distal nephron is heterogeneous with respect to function (Stoner, 1977). In this study, the initial portion of the distal tubule (diluting segment) exhibited a voltage, positive in the lumen, and a net absorption of chloride. Since the chloride was(More)
In order to determine the effect of acid lumen pH on renal tubular potassium transport, cortical collecting tubules were dissected from rabbit kidneys and perfused in vitro. When the pH of the perfusate was lowered from 7.4 to 6.8, potassium secretion into the tubule lumen decreased by an average of 47%. The transepithelial voltage increased from a mean(More)
The effects of varying endogenous aldosterone levels on the passive and active properties of the peritubular membrane were studied. Rabbits that were fed either a low Na+ (normal K+) diet or a high Na+, low K+ diet increased or decreased plasma aldosterone, respectively. Tubules were dissected, filled with oil, and incubated in 0 K+ medium to increase(More)
BURG, MI., L. STONER, J. CARDINAL, AND N. GREEN. Furosemide effect on isolated perfused tubules. Am. J. Physiol. 225(l): 119-124. 1973 .-Proximal convoluted tubules, thick ascending limbs of Henle’s loop, and cortical collecting tubules were dissected from rabbit kidneys, and perfused in vitro. The effect of the diuretic agent furosemide on electrolyte(More)
The renal diluting segment (thick ascending limb of Henle's loop) reabsorbs sodium chloride in excess of water and is responsible for dilution of the urine as well as reabsorption of a large fraction of the salt present in the glomerular ultrafiltrate. There is active reabsorption of chloride, which causes the voltage to be positive in the tubule lumen.(More)
We are able to evert and perfuse rat cortical collecting tubules (CCT) at 37 degrees C. Patch-clamp techniques were used to study high-conductance potassium channels (maxi K) on the apical membrane. Under control conditions (150 mM Na+ and 5 mM K+ in pipette and bathing solutions), the slope conductance averaged 109.8 +/- 6.6 pS (12 channels), and reversal(More)