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The colonization process of the infant gut microbiome has been called chaotic, but this view could reflect insufficient documentation of the factors affecting the microbiome. We performed a 2.5-y case study of the assembly of the human infant gut microbiome, to relate life events to microbiome composition and function. Sixty fecal samples were collected(More)
The building of a sustainable society will require reduction of dependency on fossil fuels and lowering of the amount of pollution that is generated. Wastewater treatment is an area in which these two goals can be addressed simultaneously. As a result, there has been a paradigm shift recently, from disposing of waste to using it. There are several(More)
Many of the immune and metabolic changes occurring during normal pregnancy also describe metabolic syndrome. Gut microbiota can cause symptoms of metabolic syndrome in nonpregnant hosts. Here, to explore their role in pregnancy, we characterized fecal bacteria of 91 pregnant women of varying prepregnancy BMIs and gestational diabetes status and their(More)
An upflow microbial fuel cell (UMFC) system with a U-shaped cathode inside the anode chamber was developed and produced a maximum volumetric power of 29.2 W/m3 at a volumetric loading rate of 3.40 kg COD/(m3 day) and an operating temperature of 35 degrees C while feeding sucrose continuously. The Coulombic efficiency decreased from 51.0% to 10.6% with the(More)
The upflow microbial fuel cell (UMFC) was developed to generate electricity while simultaneously treating wastewater. During a five-month period of feeding a sucrose solution as the electron donor, the UMFC continuously generated electricity with a maximum power density of 170 mW/m2. To achieve this power density, the artificial electron-mediator(More)
Taxonomic classification of the thousands-millions of 16S rRNA gene sequences generated in microbiome studies is often achieved using a naïve Bayesian classifier (for example, the Ribosomal Database Project II (RDP) classifier), due to favorable trade-offs among automation, speed and accuracy. The resulting classification depends on the reference sequences(More)
Indoor warm-water therapy pool workers in a Midwestern regional hospital were diagnosed with non-tuberculosis pulmonary hypersensitive pneumonitis and Mycobacterium avium infections. In response, we conducted a multiseason survey of microorganisms present in this therapy pool water, in biofilms associated with the pool containment walls, and in air(More)
We investigated bacterial and archaeal community structures and population dynamics in two anaerobic bioreactors processing a carbohydrate- and sulfate-rich synthetic wastewater. A five-compartment anaerobic migrating blanket reactor (AMBR) was designed to promote biomass and substrate staging, which partially separates the processes of methanogenesis and(More)
A sediment-type self-sustained phototrophic microbial fuel cell (MFC) was developed to generate electricity through the synergistic interaction between photosynthetic microorganisms and heterotrophic bacteria. Under illumination, the MFC continuously produced electricity without the external input of exogenous organics or nutrients. The current increased in(More)