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Genome-Scale Identification of Nucleosome Positions in S. cerevisiae
TLDR
A tiled microarray approach was developed to identify at high resolution the translational positions of 2278 nucleosomes over 482 kilobases of Saccharomyces cerevisiae DNA, including almost all of chromosome III and 223 additional regulatory regions, strongly suggesting that nucleosome positioning is a global determinant of transcription factor access. Expand
An Actin-Based Wave Generator Organizes Cell Motility
TLDR
It is proposed that cell motility results from the collective behavior of multiple self-organizing waves, such as the ability of cells to flow around barriers and the intricate spatial organization of protrusion at the leading edge of a motile immune cell, the human neutrophil. Expand
Spontaneous Cell Polarization Through Actomyosin-Based Delivery of the Cdc42 GTPase
TLDR
An assay in yeast where expression of an activated form of Cdc42, a Rho-type guanosine triphosphatase (GTPase) required for cell polarization, could generate cell polarity without any recourse to a preestablished physical cue was used. Expand
Membrane Tension Maintains Cell Polarity by Confining Signals to the Leading Edge during Neutrophil Migration
TLDR
It is suggested that tension, rather than diffusible molecules generated or sequestered at the leading edge, is the dominant source of long-range inhibition that constrains the spread of the existing front and prevents the formation of secondary fronts. Expand
Genomic characterization reveals a simple histone H4 acetylation code.
TLDR
Yeast strains carrying all 15 possible combinations of mutations among lysines 5, 8, 12, and 16 to arginine in the histone H4 tail are constructed, mimicking positively charged, unacetylated lysine states, and the resulting genome-wide changes in gene expression are characterized by using DNA microarrays. Expand
A Panoramic View of Yeast Noncoding RNA Processing
TLDR
A global view of yeast noncoding RNA processing is presented and it is confirmed that many currently uncharacterized yeast proteins are involved in biogenesis of nonc coding RNA. Expand
Multidimensional Drug Profiling By Automated Microscopy
TLDR
This method successfully categorized blinded drugs and suggested targets for drugs of uncertain mechanism with a titration-invariant similarity score (TISS) and is useful for discovering the mechanism and predicting the toxicity of new drugs. Expand
Endocytosis Optimizes the Dynamic Localization of Membrane Proteins that Regulate Cortical Polarity
TLDR
A mathematical model for characterizing the morphology of dynamically polarized protein distributions is constructed and predicts that polarized regions are defined with a precision that is nearly optimal for measured endocytosis rates and that polarity can be dynamically stabilized through positive feedback with directed transport. Expand
On the spontaneous emergence of cell polarity
TLDR
This work identifies an intrinsic stochastic mechanism through which positive feedback alone is sufficient to account for the spontaneous establishment of a single site of polarity, and finds that the polarization frequency has an inverse dependence on the number of signalling molecules: the frequency of polarization decreases as theNumber of molecules becomes large. Expand
Diverse drug-resistance mechanisms can emerge from drug-tolerant cancer persister cells
TLDR
The drug-tolerant persister state does not limit—and may even provide a latent reservoir of cells for—the emergence of heterogeneous drug-resistance mechanisms, including the most commonly observed clinical resistance mechanisms. Expand
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