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A high-throughput and cost-effective single-nucleotide polymorphism (SNP) genotyping method based on a gold magnetic nanoparticle (GMNP) array with dual-color hybridization has been designed. Biotinylated single-strand polymerase chain reaction (PCR) products containing the SNP locus were captured by the GMNPs that were coated with streptavidin. The GMNP(More)
A sensitive and selective biosensor platform suited for SNP type using Fe@Au magnetic nanoparticles (GMNPs) to fabricate bead array is described. This new platform integrates the rapid binding kinetics of magnetic nanoparticles carriers, the multiplexing and encoding capabilities of chips, and tagged array. As a DNA sensor, the biotinylated single-stranded(More)
To fulfill the increasing need for large-scale genetic research, a high-throughput and automated SNPs genotyping method based on gold coated magnetic nanoparticles (GMNPs) array and dual-color single base extension has been designed. Biotinylated single base extension primer were captured onto the GMNPs coated with streptavidin. Dual-color fluorescence(More)
We designed a novel automated MagStation for high-throughput single nucleotide polymorphism (SNP) discrimination using dual-color hybridization. Magnetic particles (MPs) were used as target DNA carriers. The principle for SNP discrimination in this study was allele specific hybridization using dual-color fluorescent (Cy3, Cy5) probes. The MagStation is(More)
To fulfill the increasing need for large-scale genetic research, we have developed a new solid-phase single base extension (SBE) protocol on magnetic nanoparticles (MNPs) for multiplex SNP detection using adapter polymerase chain reaction (PCR) products as templates. Extension primers were covalently immobilized on the MNPs, and allele-specific extension(More)
The performance of the cochlear implant (CI) deteriorates rapidly in the presence of background noise, and the conventional spectral subtraction speech enhancement method used in the CI can not remove the non-stationary noise well. In order to improve the audio perception ability of the CI users, an improved spectral subtraction method is proposed, which(More)
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